Objective:To investigate the effects of Compound Fufangteng Mixture(CFM)on the expression levels of CD11b^(+)monocytes surface activation molecules CD2,CD69 and depleted molecules PD-1 and CD95 in spleen and peripheral blood of immunosuppressed mice.Methods:30 healthy SPF Balb/c male mice were randomly divided into Control,immunosuppressive model group(CTX group),high,medium and low dose intervention group(CTX+CFM-H group,CTX+CFM-M group,CTX+CFM-L group),Astragalus particles(AP)intervention group(CTX+AP group).The mice in each intervention group were injected intraperitoneally with cyclophosphamide(CTX)70 mg/kg for 3 consecutive days,and then administered for 10 d according to the group regimen,and the materials were taken 24 h after the last dose.Flow cytometry detected the expression of CD11b^(+)monocytes surface activation molecules(CD2,CD69)and depleting molecules(PD-1,CD95)in mouse peripheral blood.Results:Compared with the control group,the expression levels of CD11b^(+)and CD69+in the spleen of CTX+CFM-H,CTX+CFM-M and CTX+CFM-L groups showed an improvement trend,and decreased with the dose gradient of CFM.Conclusion:CFM could regulate the immune function of splenic CD11b+ CD69 cells in immunosuppressed mice.
目的分析子宫内膜自然杀伤(natural killer,NK)细胞与女性不明原因不孕症(unexplained infertility,UI)的关系。方法随机选取并收集2022年10月至2023年1月在吉林大学第二医院行宫腔镜手术的不孕症女性部分子宫内膜组织作为研究组,并随机收集同期健康已婚已育女性的适量子宫内膜组织作为对照组,使用流式细胞术检测子宫内膜中CD56+CD3-NK细胞和CD16^(+)CD69^(+)NK细胞的百分比,采用SPSS 26.0、Medcalc统计学软件对数据进行分析。结果(1)两组患者的年龄、月经周期等一般临床资料比较,差异均无统计学意义(P>0.05)。(2)研究组与对照组CD56+CD3-NK细胞的百分比为[20.42%(13.63%,41.58%)vs 8.20%(5.83%,9.15%)],研究组与对照组CD16^(+)CD69^(+)NK细胞的百分比为[45.30%(22.20%,72.83%)vs 10.75%(3.58%,17.28%)],差异有统计学意义(P<0.001)。(3)CD16^(+)CD69^(+)NK(%)诊断UI时的曲线下面积(area under the curve,AUC)为0.862(95%CI:0.735-0.943),灵敏度为78.95%,特异度为100.00%(P<0.001)。结论子宫内膜中CD16^(+)CD69^(+)NK(%)细胞百分比的增加与不孕症的发生具有相关性,它的上调预示着某些患者局部免疫的激活,可以将其作为一个治疗靶点。
