Considerable evidence has revealed that essential oils and their main constituents possess anti-depressant and anxiolytic properties.In the current study,we report the effect ofβ-citronellol,the main component of rose essential oil,on depressive-like and anxiety-like behaviors in chronic restraint stress(CRS)mice.We found that chronic inhalation ofβ-citronellol for 14 days could increase locomotor activity in the open field test,decrease the percentage of immobility duration in the forced swimming test,and increase open arms exploration in elevated plus-maze test in CRS mice.Western blot experiment shows that chronicβ-citronellol inhalation rescues parvalbumin(PV)expression loss in the prefrontal cortex(PFC)of CRS mice.Correlation analysis reveals a strong relationship between the PV expression in PFC and the percentage of sucrose preference of the mice.These findings indicate the relationships between the PV gene expression of PFC and the effects ofβ-citronellol inhalation.
目的考察香茅醇(citronellol,CT)对人喉癌上皮细胞HEp-2和人乳腺癌细胞MCF-7增殖能力的影响,并制备CT自乳化递送系统(self-emulsified drug delivery system,SMs),对HEp-2体外抗肿瘤活性和细胞摄取能力进行评价。方法采用MTT法考察CT对HEp-2和MCF-7细胞增殖能力的影响;通过伪三元相图法优化香茅醇自乳化递送系统(CT-SMs)处方,进行外观形态、粒径和Zeta电位表征。采用MTT法检测CT-SMs对HEp-2细胞增殖能力的影响;荧光倒置显微镜定性和流式细胞仪定量考察HEp-2细胞对CT-SMs的摄取情况。结果经一定质量浓度CT处理后,MCF-7细胞增殖未受影响,差异无统计学意义(与对照组相比,P>0.05),而HEp-2细胞的增殖能力受到明显抑制(与对照组相比,P<0.05),呈剂量-时间依赖性;CT-SMs最佳处方是K_m(乳化剂与助乳化剂比例)为Kolliphor~?HS15-无水乙醇7∶3,CT-Km为3∶7。制备的微乳平均粒径为(354.0±9.5)nm,外观圆整呈类球形,分布均匀,Zeta电位为(-13.4±0.3)m V。细胞摄取实验结果表明,相同质量浓度的CT-SMs和CT处理HEp-2细胞后,CT-SMs较CT摄取量更多,分别为545.70±11.56、230.00±17.76。结论。采用滴加水法成功制备了CT-SMs,其处方工艺可行,制得的自微乳质量稳定可控。CT-SMs可明显抑制HEp-2细胞的增殖。
