搜索到2139篇“ NICOTIANA“的相关文章
Structural and Functional Insights into an Arabidopsis NBS-LRR Receptor in Nicotiana benthamiana
2024年
Nucleotide-binding site leucine-rich repeat receptors (NBS-LRR/NLRs) are crucial intracellular immune proteins in plants. Previous article reported a novel NLR protein SUT1 (SUPPRESSORS OF TOPP4-1, 1), which is involved in autoimmunity initiated by type one protein phosphatase 4 mutation (topp4-1) in Arabidopsis, however, its role in planta is still unclear. This study employed Nicotiana benthamiana, a model platform, to conduct an overall structural and functional analysis of SUT1 protein. The transient expression results revealed that SUT1 is a typical CNL (CC-NBS-LRR) receptor, both fluorescence data and biochemical results showed the protein is mainly anchored on the plasma membrane due to its N-terminal acylation site. Further truncation experiments announced that its CC (coiled-coil) domain possessed cell-death-inducing activity. The outcomes of point mutations analysis revealed that not only the CC domain, but also the full-length SUT1 protein, whose function and subcellular localization are influenced by highly conserved hydrophobic residues. These research outcomes provided favorable clues for elucidating the activation mechanism of SUT1.
Jianzhong HuangXiuying GuanXiaoju ZhongPeng JiaHongbin ZhangHonglei Ruan
The Application of Nicotiana benthamiana as a Transient Expression Host to Clone the Coding Sequences of Plant Genes
2024年
Coding sequences (CDS) are commonly used for transient gene expression, in yeast two-hybrid screening, to verify protein interactions and in prokaryotic gene expression studies. CDS are most commonly obtained using complementary DNA (cDNA) derived from messenger RNA (mRNA) extracted from plant tissues and generated by reverse transcription. However, some CDS are difficult to acquire through this process as they are expressed at extremely low levels or have specific spatial and/or temporal expression patterns in vivo. These challenges require the development of alternative CDS cloning technologies. In this study, we found that the genomic intron-containing gene coding sequences (gDNA) from Arabidopsis thaliana, Oryza sativa, Brassica napus, and Glycine max can be correctly transcribed and spliced into mRNA in Nicotiana benthamiana. In contrast, gDNAs from Triticum aestivum and Sorghum bicolor did not function correctly. In transient expression experiments, the target DNA sequence is driven by a constitutive promoter. Theoretically, a sufficient amount of mRNA can be extracted from the N. benthamiana leaves, making it conducive to the cloning of CDS target genes. Our data demonstrate that N. benthamiana can be used as an effective host for the cloning CDS of plant genes.
Jianzhong HuangPeng JiaXiaoju ZhongXiuying GuanHongbin ZhangHonglei Ruan
High-quality assembled and annotated genomes of Nicotiana tabacum and Nicotiana benthamiana reveal chromosome evolution and changes in defense arsenals被引量:1
2024年
Nicotiana tabacum and Nicotiana benthamiana are widely used models in plant biology research.However,genomic studies of these species have lagged.Here we report the chromosome-level reference genome assemblies for N.benthamiana and N.tabacum with an estimated 99.5%and 99.8%completeness,respec-tively.Sensitive transcription start and termination site sequencing methods were developed and used for accurate gene annotation in N.tabacum.Comparative analyses revealed evidence for the parental origins and chromosome structural changes,leading to hybrid genome formation of each species.Interestingly,theantiviral silencinggenesRDR1,RDR6,DCL2,DCL3,andAGO2were lost from one or both subgenomes in N.benthamiana,while both homeologs were kept in N.tabacum.Furthermore,the N.benthamiana genome encodes fewer immune receptors and signaling components than that of N.tabacum.These find-ings uncover possible reasons underlying the hypersusceptible nature of N.benthamiana.We developed the user-friendly Nicomics(http:/lifenglab.hzau.edu.cn/Nicomics/)web server to facilitate better use of Nicotiana genomic resources as well as gene structure and expression analyses.
Jubin WangQingling ZhangJeffrey TungXi ZhangDan LiuYingtian DengZhendong TianHuilan ChenTaotao WangWeixiao YinBo LijZhibing LaiSavithramma P.Dinesh-KumarBarbara BakerFeng Li
关键词:GENOMICS
黄花烟传入中国问题再研究
2024年
烟草传入问题,一直是史学界烟草史研究的重点,但以往研究主要围绕红花烟展开,黄花烟探讨存在明显不足。事实上,黄花烟亦是人们种植吸用的烟草品种之一,它的传入对当时乃至后世社会产生了深远影响。本文基于对方志、文集等文献的搜集与整理,说明崇祯年间黄花烟即已传入的可能性很大,最晚不会晚于康熙年间。传入中国主要通过走私、正常贸易及使团携传等方式,其中中亚商人、俄国私商及使团、蒙古诸部等都发挥了不同程度的积极作用。
宋娟陈青松
烟草NtPRR37基因克隆及功能分析
2024年
【目的】伪答应基因家族(pseudo response regulators,PRRs)是高等植物调控开花途径的重要基因。克隆烟草NtPRR37基因并分析其对不同光周期的应答及对开花的影响,为烟草开花调控提供靶标基因。【方法】利用同源克隆方法从普通烟草(Nicotiana tabacum L.)中克隆得到NtPRR37基因,并对其进行生物信息学分析,利用实时荧光定量PCR(real-time quantitative PCR,RT-qPCR)分析其在不同组织中的表达及不同光照时长处理的表达模式。同时利用病毒诱导基因沉默(virus induced gene silence,VIGS)技术降低NtPRR37表达水平并观察表型变化及检测开花相关基因表达变化。【结果】NtPRR37基因全长2472 bp,编码823个氨基酸,相对分子质量90.16 kD,含有PRRs基因家族的典型保守结构域(REC和CCT结构域)。通过同源进化分析发现,烟草NtPRR37与绒毛烟草(Nicotiana tomentosiformis)、林烟草(Nicotiana sylvestris)及本氏烟草(Nicotiana benthamiana)的PRR37在进化上属于同一分支。采用RT-qPCR分析发现,该基因在盛花期烟草各个组织的表达特征存在差异性,在雌蕊中的表达量最高,在侧根的表达量最低;在不同光照时长处理下,NtPRR37随着光照时间的增加表达量呈上升趋势,全黑暗处理下表达量最低,且具有生物节律性;NtPRR37沉默植株中NtPRR37表达量明显下调且沉默植株开花期提前,这可能与诱导开花相关基因(NtFT4、NtAP1、NtCO、NtSOC1)表达量显著上调有关。【结论】NtPRR37的表达受到光周期的调控,且在烟草开花过程中NtPRR37作为开花抑制因子存在。
李亦君杨小贝夏琳罗朝鹏徐馨杨军宁黔冀武明珠
关键词:烟草基因沉默开花
超表达JcFT基因对烟草农艺性状的影响
2024年
FT(Flowering locus T)基因编码的成花素蛋白,也称蛋白激素,在植物中超表达该基因往往可以显著提早开花,是创制早熟植物种质资源的重要潜在基因之一,影响植物生长发育。本研究以烟草为材料,分析了FT对烟草多个农艺性状的影响。结果表明,JcFT(FT of Jatruopha curcas L.)超表达在提早烟草开花的同时,显著改变了烟草根、茎、叶的大小和形态,而花的结构没有显著改变。
卢佳慧郑冬超张祎昕王文琪李昊阳张综博吴军
关键词:成花素超表达烟草麻疯树
本氏烟NbGAD1蛋白的原核表达及多克隆抗体制备
2024年
γ-氨基丁酸(GABA)作为一种非蛋白质氨基酸,普遍存在于植物体内,在植物生长发育和抗胁迫方面发挥着重要作用。谷氨酸脱羧酶(GAD)是合成GABA的关键蛋白酶,目前关于GAD介导GABA调控植物防御病毒侵染的分子机制尚不清楚。利用RT-PCR克隆本氏烟NbGAD1基因,并与原核表达载体pET-28a连接,构建重组载体pET-28a-NbGAD1,诱导产生重组蛋白,经Ni-NTA柱纯化蛋白后免疫新西兰白兔,最终获得抗血清。结果表明:成功构建原核表达载体pET-28a-NbGAD1,经大肠埃希菌诱导纯化后获得分子量约57 ku的重组蛋白NbGAD1。免疫制备的抗血清经Western blot检测显示,抗血清效价达1∶100000,抗血清稀释2000倍仍能检测到浓度为0.005 mg·mL^(-1)的重组蛋白,且能检测到本氏烟中内源表达的NbGAD1蛋白,说明该抗血清特异性强、灵敏度高,为今后深入探究GAD在植物病毒侵染过程中的作用机制奠定了基础。
陈龙发李凯楠王晓雯曹梦瑶陈曜朱峰
关键词:Γ-氨基丁酸谷氨酸脱羧酶原核表达抗血清制备
烟草西柏烷二萜醇类突变体的理化特征及其突变机制解析
2024年
以烟草突变体及其野生型为材料,通过测定农艺性状、腺毛密度、西柏烷二萜醇及其代谢前体物含量、西柏烷二萜醇类代谢关键基因的表达量,分析了突变体的生理生化和代谢差异,并结合基因表达差异初步解析了其突变的生理与分子机制。结果表明,突变体西柏三烯一醇和西柏三烯二醇含量分别是野生型的2.63倍和4.03倍,均极显著高于野生型;突变体分泌型腺毛密度为757.38条/cm 2,极显著大于野生型;突变体西柏烷二萜醇类代谢前体物IPP和GGPP分别为849.83 ng/g、298.29 ng/g,与野生型间的差异不显著;突变体西柏三烯一醇合成酶基因NtCYC-1、西柏三烯二醇合成酶基因NtCYP71D16的表达量分别是野生型的17.77倍和20.89倍,均极显著高于野生型。研究表明,突变体是一个培育优质、抗虫烤烟新品种的优良材料;其发生突变的原因是NtCYC-1、NtCYP71D16基因发生表达上调,促进了西柏三烯一醇、西柏三烯二醇的合成反应,同时,突变体的分泌型腺毛密度更高,为西柏烷二萜醇类的合成提供了更多的场所,使得西柏烷二萜醇类的含量显著提高。
蒙骏崔帅陆安彬龙本山陈刚刘仁祥
关键词:烟草腺毛蚜虫
转基因本氏烟草中CtDXR基因拷贝数及耐盐耐高温功能的初步分析
2024年
评估转基因本氏烟草中CtDXR基因的拷贝数,并初步分析其耐盐与耐高温能力。首先利用Southern Blot技术确定野生型本氏烟草中Actin基因的拷贝数,并以Actin基因为内参基因,以转CtDXR基因本氏烟草为材料,采用实时荧光定量PCR方法,检测转基因植株中CtDXR基因的拷贝数。最后,初步分析转基因植株的耐盐与耐高温能力。基于PCR方法,随机检测10株T1代转基因本氏烟草植株均能扩增出目的条带,表明它们均已成功转入目的基因CtDXR;Actin基因在本氏烟草基因组中为单拷贝基因;以Actin基因为内参基因,最终确定80%的转CtDXR基因植株为单拷贝或低拷贝株系;盐胁迫下,转CtDXR基因植株的株高(P<0.01)、侧根数(P<0.01)与鲜重(P<0.001)优于野生型植株,高温胁迫下,转CtDXR基因植株的株高(P<0.01)、叶片数(P<0.01)与鲜重优于野生型植株,表明转基因植株具有更强的耐盐和耐高温能力。利用研究建立的转基因本氏烟草外源基因拷贝数的检测方法,对转CtDXR基因植株完成外源基因拷贝数的鉴定,且初步鉴定了CtDXR基因具有耐盐与耐高温功能。
田春尧冀慧玥丁润月郑乔木周嘉裕廖海
关键词:拷贝数
A vicinal oxygen chelate protein facilitates viral infection by triggering the unfolded protein response in Nicotiana benthamiana
2024年
Vicinal oxygen chelate(VOC)proteins are members of an enzyme superfamily with dioxygenase or non-dioxygenase activities.However,the biological functions of VOC proteins in plants are poorly understood.Here,we show that a VOC in Nicotiana benthamiana(NbVOC1)facilitates viral infection.NbVOC1 was significantly induced by infection by beet necrotic yellow vein virus(BNYVV).Transient overexpression of NbVOC1 or its homolog from Beta vulgaris(BvVOC1)enhanced BNYVV infection in N.benthamiana,which required the nuclear localization of VOC1.Consistent with this result,overexpressing NbVOC1 facilitated BNYVV infection,whereas,knockdown and knockout of NbVOC1 inhibited BNYVV infection in transgenic N.benthamiana plants.NbVOC1 interacts with the basic leucine zipper transcription factors bZIP17/28,which enhances their self-interaction and DNA binding to the promoters of unfolded protein response(UPR)-related genes.We propose that bZIP17/28 directly binds to the NbVOC1 promoter and induces its transcription,forming a positive feedback loop to induce the UPR and facilitating BNYVV infection.Collectively,our results demonstrate that NbVOC1 positively regulates the UPR that enhances viral infection in plants.
Zhihong GuoNing JiangMenglin LiHongfang GuoQi LiuXinyu QinZongying ZhangChenggui HanYing Wang

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李立芹
作品数:242被引量:333H指数:10
供职机构:四川农业大学
研究主题:马铃薯 烟草 克隆 基因 基因表达
鲁黎明
作品数:348被引量:561H指数:12
供职机构:四川农业大学农学院
研究主题:烟草 烤烟 马铃薯 生物信息学 烟叶
姚恒
作品数:148被引量:102H指数:6
供职机构:云南省烟草农业科学研究院
研究主题:烟草 雪茄烟 克隆 烟叶 烟草植株
张建奎
作品数:109被引量:672H指数:17
供职机构:西南大学
研究主题:小麦 烤烟 育性 育性转换 AESTIVUM
杨焕文
作品数:197被引量:1,758H指数:24
供职机构:云南农业大学烟草学院
研究主题:烤烟 烟草 烟叶 产质量 漂浮育苗