目的探讨慢性阻塞性肺疾病急性加重期(AECOPD)患者平面细胞极性(Wnt/PCP)信号通路中Ras homolog gene family member A(RhoA)蛋白表达与中医证型的相关性,尝试为AECOPD中医证型诊断提供一个客观标准。方法收集痰浊壅肺证、痰热郁肺证、痰蒙神窍证、肺肾气虚证、阳虚水泛证5个证型的AECOPD患者各20例。健康组15例。所有入选患者空腹抽取静脉血,采用Enzyme Linked Immunosorbent Assay(ELISA)检测五个组别血清中RhoA蛋白量,并探讨其相关性。结果与健康组比较,AECOPD患者5个证型的RhoA蛋白量明显增高,具有显著统计学意义(P<0.05);五个中医证型血清中RhoA蛋白量比较:痰浊壅肺组<痰热郁肺组<痰蒙神窍组<肺肾气虚组<阳虚水泛组。RhoA蛋白表达量与AECOPD五组中医证型之间存在直线线性相关关系,且呈正性相关。结论 AECOPD患者Wnt/PCP信号通路中RhoA蛋白量在五个中医证型中存在显著差异,能够给AECOPD中医证型诊断提供一个客观标准,并揭示其疾病严重程度。
To investigate the mechanism of effect of Huanglian Jiedu Decoction(HLJD) on vascular endothelium, we assessed the protective effect of HLJD on vascular endothelium in spontaneously hypertensive rats(SHR) and the expression of RhoA in thoracic aorta. A total of 40 SHR rats were randomly and evenly divided into model group(SHR group), positive group(captopril group), HLJD high-dose group, and HLJD low-dose group. Simultaneously, 10 Wistar Kyoto rats were used in the blank group. All groups were treated by gavage for 6 weeks. The changes of nitric oxide synthase(NOS), von Willebrand factor(vWF), endothelin(ET-1) and calmodulin(CAM) in rat serum were tested by enzyme linked immunosorbent assay(ELISA) method. The expression of RhoA at the protein and mRNA levels in thoracic aorta was determined by Western blotting(WB) and quantitative real-time PCR, respectively. Compare with the blank group after 6 weeks, the levels of ET-l and VWF in serum of the model group were significantly increased(P<0.01), and the levels of NOS and CAM were significantly decreased(P<0.01). Conversely, the levels of ET-1 and vWF in the positive and HLJD groups were significantly decreased(P<0.01 or P<0.05), and the levels of NOS and CAM were significantly increased(P<0.01 or P<0.05) compared with the model group. The expression of Rho A at the mRNA and protein levels was decreased obviously(P<0.05) in HLJD high-dose group. The results shown that HLJD increased diastolic factor(CAM and NOS) in the vascular endothelial of rats, leading to reduced contraction factor(ET-1 and vWF). HLJD revealed the preventive function in the vascular endothelial dysfunction of the early stage hypertension through adjusting secretion of blood vessel endothelium(BVE) relaxing factor and improving vascular endothelial function. The mechanism might be associated with the inhibition of the activity of RhoA protein factor.
