[Objective] The aim was to study the flavonoid content of Fagopyrum megaspartanium leaves by water bath and ultrasonic extractions.[Method] By using water bath extraction and ultrasonic extraction,the total flavonoid content in buckwheat leaves was measured with UV spectrophotometer.[Result] The optimum wavelength for determination of the content of flavonoid extracted from buckwheat leaves was 420 nm;the content of total flavonoid extracted by ultrasonic extraction and water bath extraction from buckwheat were 5.26% and 5.40% respectively;and the average total flavonoid content by water bath extraction was significantly higher than that by ultrasonic extraction.[Conclusion] The result is significant for the improvement of flavonoid content measurement of buckwheat.
[Objective]The study aimed to carry out RAPD analysis of genetic diversity of common and tartary buckwheat varieties.[Method] The genetic diversity of 19 common and tartary buckwheat varieties including the tested varieties in Guizhou region during 1999-2010 and their parents were studied using 7 primers by means of random amplified polymorphic DNA（RAPD）.[Result]A total of 149 DNA bands were obtained.In which,141 bands were polymorphic,accounting for 94.89%.Polymorphism analysis and cluster analysis showed that all varieties had their own special bands different from each other.The varieties native to Weining were close to each other,and other common buckwheat varieties were obviously different from each other.The interspecific genetic variation was the greatest;the intraspecific genetic variation of common buckwheat varieties was greater than that of tartary buckwheat varieties.[Conclusion]The RAPD fingerprints of the 19 buckwheat varieties were established in present study.
[Objective] The paper aimed to explore a simple in situ PCR technology for buckwheat.[Method] By using 16S and 4.5S nested primers and psbA primer,the in situ PCR,nested in situ PCR,and multiple in situ PCR were carried out on common buckwheat,respectively.[Result] High-temperature drying treatment had the effects similar to that of embedding method.The effect of the nested in situ PCR is better than conventional in situ PCR.A better result could be obtained till the multiple in situ PCR was performed as many as 5-6 times.Four pair of signals could be obtained by using both 16S and 4.5S primers,but their sites differed from each other;psbA primer as a single copy only showed a pair of signals.A total of five pairs of common buckwheat chromosome could be identified according to the difference of the signal＇s location.[Conclusion] The chromosome in situ PCR technique for buckwheat was simple and feasible.
为丰富普通荞麦（Fagopyrum esculentum Moench）的序列信息，挖掘有效的 SSR 标记，基于Hiseq 2000测序平台对普通荞麦的种子转录谱进行测序、分析，利用 Misa 软件进行 SSR 位点扫描，采用Primer 5．0引物设计程序对其中的300个 SSR 位点设计引物，随机挑选40对引物对19个普通荞麦品系进行遗传多样性分析。结果表明：测序共产生20508824条高质量的短序列（read），总长度为1889111004 bp，通过拼接最终获得54947条转录本（transcript）和36133个独立基因（unigene）。在2226个独立基因中发现了2666个 SSR 位点。有20对引物（占50％）能扩增出目标产物，其中，12对有多态性，多态性信息量（PIC）范围为0．10～0．93，平均为0．57，多态性程度高。
