Sensing environmental temperature is crucial for animal life.The model animal,Drosophila melanogaster,can be investigated with a large number of genetic tools,which have greatly facilitated studies of the cellular and molecular mechanisms of thermal sensing.At the molecular level,a group of proteins,including Transient Receptor Potential channels and ionotropic receptors,have been characterized as potential thermal sensors in both larval and adult Drosophila.At the cellular and circuit levels,peripheral and central thermosensory neurons have been identified.More interestingly,thermal information has been found to be specifically encoded by specific central neurons.In this short review,we mainly survey the progress in understanding the molecular mechanisms of thermosensation and the neuronal mechanisms of thermal information processing in the brain of Drosophila.Other recent temperature-related findings such as its impact on neurosecretion and thermotactic behavior in Drosophila are also introduced.
The fruit fly,Drosophila melanogaster,is able to discriminate visual landmarks and form visual long-term memory in a flight simulator.Studies focused on the molecular mechanism of long-term memory have shown that memory formation requires mRNA transcription and protein synthesis.However,little is known about the molecular mechanisms underlying the visual learning paradigm.The present study demonstrated that both spaced training procedure(STP)and consecutive training procedure(CTP)would induce long-term memory at 12 hour after training,and STP caused significantly higher 12-h memory scores compared with CTP.Labelfree quantification of liquid chromatography-tandem mass spectrometry(LC-MS/MS)and microarray were utilized to analyze proteomic and transcriptomic differences between the STP and CTP groups.Proteomic analysis revealed 30 up-regulated and 27 down-regulated proteins;Transcriptomic analysis revealed 145 up-regulated and 129 down-regulated genes.Among them,five candidate genes were verified by quantitative PCR,which revealed results similar to microarray.These results provide insight into the molecular components influencing visual long-term memory and facilitate further studies on the roles of identified genes in memory formation.
When facing a sudden danger or aversive condition while engaged in on-going forward motion,animals transiently slow down and make a turn to escape.The neural mechanisms underlying stimulation-induced deceleration in avoidance behavior are largely unknown.Here, we report that in Drosophila larvae, light-induced deceleration was commanded by a continuous neural pathway that included prothoracicotropic hormone neurons, eclosion hormone neurons, and tyrosine decarboxylase 2 motor neurons(the PET pathway). Inhibiting neurons in the PET pathway led to defects in lightavoidance due to insufficient deceleration and head casting.On the other hand, activation of PET pathway neurons specifically caused immediate deceleration in larval locomotion. Our findings reveal a neural substrate for the emergent deceleration response and provide a new understanding of the relationship between behavioral modules in animal avoidance responses.
Caixia GongZhenhuan OuyangWeiqiao ZhaoJie WangKun LiPeipei ZhouTing ZhaoNenggan ZhengZhefeng Gong
Dear Editor,The innate preference behaviors of animals can be modified by external environmental conditions.In Drosophila for example,the preference for food and temperature are respectively influenced by the hardness of food and environmental light conditions[1,2].Comparatively,environmental modulation of Drosophila light preference has received less investigation.Drosophila avoids light and prefers darkness in the larval stage[3,4].Drosophila larval photoreceptors,Bolwig's organs,and downstream neurons such as the 5th-lateral neurons[4,5]and the posterior ventral lateral-09 neurons[6],are required for the lightavoidance response.
Jie WangWeiqiao ZhaoQianhui ZhaoJinrun ZhouXinhang LiYinhui HeZhefeng Gong
Innate preference behaviors are fundamental for animal survival. They actually form the basis for many animal complex behaviors. Recent years have seen significant progresses in disclosing the molecular and neural mechanism underlying animal innate preferences, especially in Drosophila. In this review, I will review these studies according to the sensory modalities adopted for preference assaying, such as vision, olfaction, thermal sensation. The behavioral strategies and the theoretic models for the formation of innate preferences are also reviewed and discussed.
Animals always seek rewards and the related neural basis has been well studied. However, what happens when animals fail to get a reward is largely unknown,although this is commonly seen in behaviors such as predation. Here, we set up a behavioral model of repeated failure in reward pursuit(RFRP) in Drosophila larvae. In this model, the larvae were repeatedly prevented from reaching attractants such as yeast and butyl acetate, before finally abandoning further attempts. After giving up, they usually showed a decreased locomotor speed and impaired performance in light avoidance and sugar preference,which were named as phenotypes of RFRP states. In larvae that had developed RFRP phenotypes, the octopamine concentration was greatly elevated, while tbh mutants devoid of octopamine were less likely to develop RFRP phenotypes, and octopamine feeding efficiently restored such defects. By down-regulating tbh in different groups of neurons and imaging neuronal activity, neurons that regulated the development of RFRP states and the behavioral exhibition of RFRP phenotypes were mapped to a small subgroup of non-glutamatergic and glutamatergic octopaminergic neurons in the central larval brain. Our results establish a model for investigating the effect of depriving an expected reward in Drosophila and provide a simplified framework for the associated neural basis.
Yue FeiDikai ZhuYixuan SunCaixia GongShenyang HuangZhefeng Gong
