Protein N-glycosylation plays very important roles in immunity and α-mannosidase is one of the key enzymes in N-glycosylation.This paper reports that inhibition of α-mannosidase Man2cl gene expression enhances adhesion of JurkatT cells.In comparison to the controls with normal expression of the enzyme,Jurkat cells with the inhibition of Man2clgene expression(AS cell)formed larger aggregates in culture,indicating an enhancement of adhesion between the cells.mRNA differential display analysis discovered up-regulation of several adhesion molecule genes in the AS cell.Becauseof the pivotal role played by CD54-LFA-1 interaction in immune cell interaction,this study focused on the contributionof enhanced expression of CD54 and LFA-1 to the enhanced adhesion of AS Jurkat cells.These facts,including increasedbinding of AS cells to ICAM-1-Fc,Mg^(2+)activation of the binding of AS cells to ICAM-1-Fc and enhanced aggregationof AS cells,together with the inhibiting effect of a blocking CD 11a mAb on the binding to ICAM-1-Fc and aggregationof the cells demonstrate an important contribution of enhanced CD54-LFA-1 interaction to increased adhesion betweenAS cells.The enhanced CD54-LFA-1 interaction also resulted in increased adhesion between AS Jurkat T cells and RajiB cells.In addition,AS cells showed cytoskeletal rearrangement.The data imply a biological significance of MAN2C1in T-cell functioning.
Li Qu Ji Yu Ju Shuang Ling Chen Yah Shi Zhi Guang Xiang Yi Qun Zhou Yun Tian Yin Liu Li Ping Zhu
