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国家自然科学基金(81271035H1205)

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芬戈莫德的非免疫抑制效应研究进展
2015年
芬戈莫德(FTY720)是一种新型的免疫抑制剂,是从冬虫夏草(子囊菌亚门赤僵菌)中提取出来的抗菌药物成分多球壳菌素(ISP-1)经过化学修饰改造而成。关于FTY720的免疫抑制效应一直是研究的热点,如FTY720在多种器官移植模型及自身免疫病、肿瘤等疾病研究中均显示了良好的效果[1-3]。
韩冰刘苏
关键词:芬戈莫德中枢神经系统
芬戈莫德对视网膜光损伤大鼠光感受器细胞及小胶质细胞的影响
2015年
目的观察芬戈莫德(FTY720)对视网膜光损伤大鼠光感受器细胞及小胶质细胞的影响。方法Sprague-Dawley大鼠120只随机分为正常组、模型组、溶媒组和FTY720组,每组30只。正常组大鼠不予处理;模型组、溶媒组和FTY720组大鼠建立光损伤动物模型。模型组大鼠仅接受光照。FTY720组大鼠建模前腹腔注射FTY720,溶媒组大鼠注射50%二甲基亚砜。光照后6h及1、3、7d,采用流式细胞仪检测大鼠小胶质细胞所占比例;酶联免疫吸附试验检测大鼠视网膜中白细胞介素(IL)-1β的含量。光照后1d,采用原位末端标记法检测光感受器细胞凋亡情况。光照后7d,采用苏木精一伊红染色观察大鼠视网膜形态结构。结果大鼠视网膜中小胶质细胞所占比例及IL-1β含量均于光照后6h开始升高,光照后3d达高峰,光照后7d开始下降。光照后6h及1、3、7d,FTY720组大鼠视网膜中小胶质细胞所占比例及IL-1β含量较正常组升高,较模型组、溶酶组明显降低,差异均有统计学意义(P〈0.05)。光照后1d,正常组、模型组、溶酶组及FTY720组大鼠视网膜凋亡细胞比例分别为0、(87.66±2.50)%、(86.00±2.44)%、(49.66±2.80)%。FTY720组大鼠视网膜凋亡细胞比例较正常组升高,较模型组、溶酶组明显降低,差异均有统计学意义(P〈0.05)。光照后7d,正常组大鼠视网膜结构清晰,细胞排列整齐;溶酶组、模型组大鼠视网膜结构不清楚,细胞排列紊乱,外核层明显变薄;FTY720组大鼠视网膜结构清晰,外核层厚度较溶酶组、模型组厚,但薄于正常组。结论FTY720能抑制视网膜光损伤大鼠光感受器细胞的凋亡及小胶质细胞的活化。
韩冰刘苏
Changed trends of major causes of visual impairment in Sichuan,China from 1987 to 2006
2014年
AIM: To study the trends of major causes of visual impairment(VI) in adults in Sichuan,China and evaluate the effect of aging on the trends. ·METHODS: We used data from the National Sample Survey on Disabilities(NSSD) in Sichuan province conducted in 1987 and 2006. The age-adjusted prevalence of major causes of VI and the prevalence stratified by age in each cause were calculated and compared. The association between age and each cause of VI was also analyzed.·RESULTS: Retinal disease increased and became the second leading cause of VI in 2006 while blinding trachoma decreased markedly. Cataract and non-trachomatous corneal diseases were among the leading causes of VI in both years. We found associations between age and causes of VI,with age showing the strongest association with cataract and relatively lower associations with other causes. · CONCLUSION: In the last two decades,dramatic changes occurred in the major causes of VI with significantly increased retinal disease and decreased blinding trachoma. Aging of the population might be an important factor accounting for the changed trends of VI. Understanding the prevalence of VI,its major causes and trends over time can assist in prioritizing and developing effective interventional strategies and monitoring their impact.
Hui ChenYing-Chuan FanQi-Hong HeXiao-Yun WuMin WeiJune E.EichnerBradley K.FarrisP.Lloyd HildebrandChun-Tao LeiShu-Hua WuJing-Yun Yang
关键词:TRACHOMACATARACTGLAUCOMA
Comparison on conjunctival sac bacterial flora of the seniors with dry eye in Ganzi autonomous prefecture被引量:4
2013年
AIM:To compare the bacterial flora in palpebral conjunctiva of xerophthalmia seniors of Tibetan,Yi and Han,and analyze the differences and similarities of the bacteria.·METHODS:The test subjects were selected from 2Tibetan,2 Yi and 3 Han populated places,respectively.Total 222 seniors(444 eyes) with dry eye were examined.Secretion was collected from the palpebral conjunctiva of the subjects and then inoculated onto a blood agar plate.After 48h of incubation,the bacteria were examined for the differences and similarities between different ethnics.·RESULTS:There was no significant difference(P >0.05)of Gram stain characterization,dominant bacteria and number of the bacterial species present in oxrophthalmia patients among Tibetan,Yi and Han nationalities.The bacteria presented in all groups include staphylococcus epidermidis,corynebacterium,micrococcus luteu,int-racellular bacteria sphingomonas,pseudomonas aerug-inosa.The bacteria detected from the two of three ethnic groups were staphylococcus aureus,staphylococcus haemolyticus,escherichia coli,kytococcus sedentarius,streptococcus angina,micrococcus lylae,and staphy-lococcus heads.The incidence rate of bacteria-associated dry eye in Tibetan population was significantly lower than that of Han and Yi population.·CONCLUSION:There is no significant difference in the bacteria flora of palpebral conjunctiva observed among dry eye elder populations of Tibetan,Yi and Han people.All of staphylococcus epidermidis,corynebacterium,micrococcus luteu,intracellular bacteria sphingomonas,pseudomonas aeruginosa,staphylococcus aureus,staphylococcus haemolyticus,escherichia coli,kytococcus sedentarius,streptococcus angina,micrococcus lylae and staphylococcus heads are common bacteria flora of the three nationalities inhibiting in this area.
Yue ZhangZhi-Rong LiuHui ChenYing-Chuan FanJi DuoHong ZhengGuang-Jin WangYu-Chan LiDan-Ba JiachuGe-Ma Zewang
关键词:CONJUNCTIVABACTERIATIBETAN
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