We used a litterbag method to investigate litter decomposition and related soil degradative enzyme activities across four seasons in a broad-leaved forest and a coniferous forest on Zijin Mountain in sub-tropical China.Across four seasons, we quantified litter mass losses,soil pH values, and related soil degradative enzyme activities.Litter decomposition rates differed significantly by season.Litter decomposition rates of broadleaf forest leaves were higher than for coniferous forests needles across four seasons, and maximal differences in litter decomposition rates between the two litter types were found in spring.Obvious differences in litter decomposition rates of the two litter types were found in winter, which were similar to rates in spring.Litter decomposition rates of the two litter types in autumn were significantly higher than in spring.Soil degradative enzyme activities were lowest in winter and highest in summer in most cases across four seasons.
WANG Cong-yanLü Yan-naWANG LeiLIU Xue-yanTIAN Xingjun
为评价撕裂蜡孔菌P2处理橙黄G染料废水的应用潜力,采用批次实验在开敞系统中研究静置与摇动、染料初始浓度、pH、温度、盐度、碳源、氮源、金属离子等因子对该菌降解橙黄G染料废水的影响,同时利用植物萌发与微生物抑菌试验进行染料与脱色溶液的毒性测试.结果表明,与摇动培养相比,静置培养更适合于撕裂蜡孔菌的脱色,最适脱色pH与温度分别为9和25℃.盐度测试结果显示撕裂蜡孔菌能在浓度为128 g L-1的盐溶液中能进行高效脱色,可达70%以上.在上述参数体系的优化基础上,分别进行了碳源、氮源与金属离子的添加优化实验,结果显示碳源、氮源与金属离子的最适浓度分别为4 g L-1葡萄糖、0.15 g L-1硝酸铵和0.1 mmol L-1 Zn2+.菌丝吸附在整个脱色过程中作用较小,撕裂蜡孔菌对橙黄G的脱色过程以酶的降解为主,未发现该菌分泌漆酶,只分泌锰过氧化物酶与木质素过氧化物酶,其最高活性分别为230 U mL-1和158 U mL-1.植物与微生物毒性分析显示撕裂蜡孔菌脱色后的产物对植物与微生物的毒性大大降低.因此,撕裂蜡孔菌对于处理橙黄G染料废水具有良好的应用潜力.
Aims With the continuing increase in the impact of human activities on ecosystems,ecologists are increasingly interested in understanding the effects of high temperature on litter decomposition since litter decomposition and the accompanying release of nutrients and carbon dioxide are key processes in ecosystem nutrient cycling and carbon flux.This study was conducted to evaluate the temperature sensitivity of forest litter decomposition and soil enzymes during litter decomposition in subtropical forest in China.Methods Two dominant litter types were chosen from Zijin Mountain in China:Quercus acutissima leaves from a broadleaf forest(BF)and Pinus massoniana needles from a coniferous forest(CF).The litter samples were incubated in soil microcosms at ambient control temperature(20 C)and 10 C warmer.During a 5-month incubation,chemical composition of litter samples,litter mass losses,and related soil enzyme activities were determined.Important Findings Three main results were found:(i)high temperature accelerated decomposition rates of both litter types,and the temperature sensitivities of litter decomposition for BF leaves and that for CF needles are equivalent basically,(ii)high temperature enhanced soil enzyme activities in the two forest types,and the temperature sensitivities of polyphenol oxidase were significantly higher than those of the other soil enzymes and(iii)the temperature sensitivities of nitrate reductase were significantly higher in the CF soil than in the BF soil,while there was no significant difference in the temperature sensitivities of the other soil enzymes between BF and CF.As a long-term consequence,the high-temperature-induced acceleration of litter decomposition rates in these subtropical forests may cause carbon stored belowground to be transferred in the atmosphere,which may alter the balance between carbon uptake and release,and then alter the global carbon cycle in the coming decades.
