In the past two decades,the biological and medical fields have seen great advances in the development of biosensors and bioehips capable of characterizing and quantifying biomolecules.This lecture is meant to discuss the development and applications of advanced electroanalysis,biophotonics,nanotechnology,MEMS- based biosensors and biochips for biomedical diagnostics and physical performances of athlete.
SPR (Surface Plasmon Resonance) is an emerging optical biosensor which can monitor the processes at metal interface in real time without labeling requirements.Instrument SPR-2004 can sense two areas in one channel and get two signals at same time in one test.One signal is the graph of the reaction;the other is the graph of reference.SPR biochip is modified with dextran,and only reaction area can be used to immobilize the protein.Two methods of antibody immobilization on chip were tested.One was to immobilize directly,the other was to immobilize protein A firstly and then the antibody was caught by immobilized protein A.The latter was chosen as suitable for retention of the native binding ability with virus of antibody.The latter chip was used to detect SARS-Cov.The signal of detection reached to 60 units within 40 min.It was 55 units even after reference.
Yujie WangDafu CuiJunbo WangHaoyuan CaiZipan ZhengWei DengChuan QinXiuhong Yang
A PDMS electrophoresis microchip,which integrated with optical fiber for fluorescence detection,was fabricated by using silicon master.A deep reactive ion etches (DRIE) technology was used to fabricate the silicon master with positive features.The PDMS replica was fabricated by casting PDMS prepolymer against the silicon master,where an optical fiber was first fixed on the end of separation microchannel.To improve the rigid characteristics of integrated PDMS microchip,the chips were subsequently assembled by reversible sealing against glass plate.A blue light emitting diode (LED) was used as excitation light sources for inducing fluorescence detection through coupling LED light into the optical fiber.As an application, integrated PDMS microchip was tested in the capillary electrophoresis separation of DNA markers.The results showed that DNA markers could be effectively separated and detected except for the segments of 271 and 281.
Changchun Liu Dafu Cui Haoyuan Cai Bo Su Xing Chen Haining Wang Zhaoxin Geng
