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Aberrant expression of the IFN regulatory factor 4-binding protein (IBP) in human colorectal cancer: Recent studies shown that the IFN regulatory factor 4-binding protein (IBP) plays multiple biological roles in...; Chuanmin Hu Zhujun Zhang Peng Li Qingliang Wang Yu Zhou Tao Fu Shuhui Li Weijing Yi Xinjun Li Yang Zhang Mingzhen Yang Xiuwu Bian Ji Ming Wang Guoguang Ying An Chen (a Department of Clinical Biochemistry Laboratory Science,Third Military Medical University,Chongqing 400038,PR China

Molecular cloning,sequence analysis and overexpression of a novel creatine amidinohydrolase gene from the Arthrobacter Nicotianae strain 02181: The creatine amidinohydrolase(creatinase)gene encoding a high activity creatinase from the Arthrobacter nicoti...; Qiang Zhi Peiyan Kong Jiatao Zang Youhong Cui Shuhui Li Peng Li Weijing Yi Shaobo Su and Chuanmin Hu (a Department of Clinical Biochemistry,Third Military Medical University,Chongqing 400038,PR China; 文献传递

抗淋巴样增强因子-1羧基端单克隆抗体的制备及其表位的初步鉴定被引量：2: 2008年; 目的制备抗淋巴样增强因子-1(LEF-1)羧基端86个氨基酸(Lct86)特异性单克隆抗体,并初步明确其表位,为进一步研究LEF-1C端特异序列的功能奠定基础。方法从Jurkat细胞株中扩增LEF-1C端特异序列cDNA,并克隆入PQE40、pET32a原核表达载体;分别转化入M15、BL21(DE3)感受态菌,IPTG诱导表达;纯化PQE40-Lct86融合蛋白作为免疫原免疫Balb/c小鼠制备单克隆抗体,以pET32a-Lct86融合蛋白鉴定单克隆抗体特异性。LEF-1C端特异序列不同长度的基因片段定向克隆入PQE40,鉴定各片段的表达,利用Western blotting初步鉴定单克隆抗体的表位。结果成功构建了PQE40-Lct86、pET32a-Lct86重组原核表达载体;纯化获得PQE40-Lct86融合蛋白;得到一株特异性的抗LEF-1C端单克隆抗体TMAb1,证实其表位位于437aa^454aa。结论成功制备了一株特异性的抗LEF-1C端单克隆抗体TMAb1,并鉴定其抗原识别表位位于437aa^454aa,为进一步研究LEF-1C端的功能奠定基础。; 杨明珍李鹏张竹君易维京李淑慧赵利军胡川闽; 关键词：单克隆抗体表位

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国家自然科学基金(30570373)