Rhodococcus sp.DS-3 with the ability to selectively desulfurize dibenzothiophene and grow with the sulfur atom desulfurized was quantitatively characterized in batch cultures.The fermentation process for desulphurization by DS-3 was analyzed.The transformation of dibenzothiophene to 2-hydroxydiphenyl was 86.3% in BioStat C10-3 fermentor with aerating at 48 L·h -1 , constant pH 7.8,fermentation temperature 30℃ and stirring at 300 r·min -1 .The kinetic models of growth,substrate consumption and product generation were established.The optimal parameters were estimated and fitted to model software MATLAB.The results, which showed the actual values in 95% confidence interval, proved that these kinetic models fitted well and fundamentally reflected the regularity of dibenzothiophene desulphurization in the fermentor.
