The multisubunit NADH ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. The iron-sulfur protein (IP) fraction is made up of 7 subunits, including NDUFS6 (NADH-ubiquinone oxidoreductase Fe-S protein 6). In order to understand the giant panda’s NDUFS6 gene and coding of protein using RT-PCR combined with in silico cloning, we isolated and sequenced the cDNA encoding NADH-ubiquinone oxidoreductase iron-sulfur protein 6 of the giant panda (Ailuropoda melanoleuca).The deduced protein sequence showed that the protein is composed of 124 amino acids and the estimated molecular weight of the NDUFS6 protein is 13.632 kDa with an isoelectric point (PI) of 9.51. Alignment analysis reveals that the deduced protein sequence shares 85.6 %,82.4 %,76.6 % and 76.1 % homology with that of Homo sapiens, Bos taurus, Rattus norvegicus and Mus musculus, respectively. In particular, there are 2 Protein kinase C phosphorylation sites (14SGK16 and51SRK53) in the NDUFS6 protein of the giant panda.
In order to provide scientific material for promulgating the giant panda(Ailuropoda melanoleuca)nucleus gene inheritance characteristic,for the formulation gene protection countermeasure and to put resources to rational use,the expression sequence of ubiquinol-cytochrome c reductase complex ubiquinone-binding protein(QP-C)gene from the giant panda was analyzed.The result indicates that the cDNA sequence length is 335 bp and contains a 246-nucleotide open reading frame encoding 81 amino acid residues.The pI of the protein is 10.50 and its mole cular weight is 9.60×103 Da.Topology prediction shows these QP-C proteins contain a N-glycosylation site,a potential protein kinase C phosphorylation site and a Casein kinase II phosphorylation site.Comparing the cDNA sequence with amino acid sequence of giant panda’s QP-C gene and cDNA sequence with the amino acid sequence of other species’ QP-C gene shows high similarity.The similarity is 82.3% to 95.2% and 81.5% to 95.1%.
