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Co-suppression of the EIN2-homology Gene LeEIN2 Inhibits Fruit Ripening and Reduces Ethylene Sensitivity in Tomato: EIN2(ethylene insensitive 2) is a very important component in ethylene signal transduction pathway. Recently,t...; Zongli Hu 1,Lei Deng 1,Xuqing Chen 1; 关键词：CO-SUPPRESSION ETHYLENE TOMATO; 文献传递

番茄中脂肪氧化酶基因D的克隆、表达及功能分析: 从番茄叶中扩增到TomloxD全长基因,该片段包含1个2727 bp的开放阅读框,编码一条由909个氨基酸组成的多肽链。将TomloxD基因构建到酵母分泌表达载体pPIC9K上,电转化入巴斯德毕赤酵母(Pichia pa...; 屈霄霄胡廷章胡宗利陈国平; 关键词：巴斯德毕赤酵母机械损伤脂肪氧化酶; 文献传递

Silencing LeSGR1 gene in tomato inhibits chlorophyll degradation and exhibits a stay-green phenotype: The full-length cDNA of LeSGR1 was cloned from tomato by RT-PCR and RACE.The cDNA encoded a protein of 272 ami...; Zongli Hu 1,Lei Deng 1,Bo Yan 1,Yu Pan 1,Min Luo 1,Xuqing Chen 2,Tingzhang Hu 1 and Guoping Chen 1 (1 Key Laboratory of Biorheological Science and Technology,Ministry of Education,Bioengineering College, Chongqing University,Chongqing 400044; 关键词：TOMATO STAY-GREEN SENESCENCE; 文献传递

蜂毒肽融合蛋白在裂殖酵母中的表达研究: 将抗菌肽之一的蜂毒溶血肽(Melittin)基因序列定向克隆到表达载体pESP-2中,构建GST-Melittin融合蛋白。利用载体上严格受硫胺素浓度调控的启动子Pnmtl,实现融合蛋白在裂殖酵母Schizosaccha...; 胡宗利刘志昭邓磊潘宇陈国平; 关键词：蜂毒肽裂殖酵母蛋白表达; 文献传递

One-Step Purification of Functional Light-harvesting 2 Complexes from Rhodobacter sphaeroides: Most common method for isolation of light-harvesting 2 complexes(LH2) is very time-consuming. Functional LH2 c...; Zhiping Zhao,Zongli Hu,Yan Liang,Tingzhang Hu,Yun Tu,Guoping Chen (Key Laboratory of Biorheological Science and Technology,Ministry of Education,Bioengineering College,Chongqing University,Chongqing 400044); 关键词：HIS-TAG; 文献传递

红菜薹春化相关基因的克隆与表达被引量：1: 2010年; 红菜薹是一种不经春化处理就可开花的天然早花突变体.根据已报道的拟南芥和芸薹属植物春化相关基因的保守区域设计引物,通过PCR和RT-PCR方法从红菜薹中克隆得到了4个春化相关的关键基因片段:BrFRI、BrFLC、BrVIN3、BrSOC1,获得BrFRI起始密码子附近的关键序列,其中BrVIN3基因序列为首次报道,含有PHD区关键区域.Northern和半定量RT-PCR表达分析表明:BrFRI的表达不受春化作用的影响,BrFLC受春化作用的抑制,BrVIN3和BrSOC1受春化作用的诱导.; 陈国平胡功铃胡宗利李勇顾峰; 关键词：红菜薹春化相关基因克隆

Expression of OsLEA3 gene enhanced the tolerance to abiotic stresses in E.coli and Rice: The OsLEA3 is a late embryogenesis abundant group 3 protein.The OsLEA3 gene was cloned from rice seeding which...; Tingzhang Hu,Xiaoxiao Qu,Zongli Hu,Shuai He,Guoping Chen (Bioengineering College,Chongqing University,Chongqing 400044); 关键词：RICE; 文献传递

火焰原子吸收光谱法测定芦荟中微量铁、锌含量被引量：2: 2011年; 用火焰原子吸收光谱法测定了芦荟中铁、锌两种微量元素含量。结果表明,芦荟中铁和锌干含量分别为638、244μg/g。火焰原子吸收光谱法测定样品中铁含量和锌含量的相对标准偏差分别为7.06%,3.63%,加标回收率在95%～102.5%范围内,检测限分别为0.011μg/mL和0.002μg/mL。; 叶梅朱兴琼; 关键词：火焰原子吸收光谱法芦荟锌

植物叶色白化研究进展被引量：33: 2012年; 植物叶色白化是一类明显和常见的叶绿素缺失突变,在植物光合作用机理、激素生理、核-质基因组相互作用、遗传育种等理论研究和实际应用方面均具有无可替代的价值。主要综述了近年来国内外有关白化突变体与叶绿素合成的关系、影响白化的内外因素、相关基因的克隆以及克服白化的途径等方面的研究进展,旨在为深入研究植物叶色白化突变提供参考。; 朱明库胡宗利周爽李亚丽陈国平; 关键词：白化叶绿素影响因素基因克隆

Cloning and Functional analysis of LeMBF1 involved in abiotic and biotic stress response in tomato: The full-length cDNA of LeMBF1 was cloned from tomato by RT-PCR.The cDNA encoding an open reading frame of 139...; Zongli Hu 1,Lei Deng 1,Dafei Wang 1,Yu Pan 1,Yang Liu 1,Xuqing.Chen 2,Tingzhang Hu 1 and Guoping Chen 1 (1 Key Laboratory of Biorheological Science and Technology,Ministry of Education,Bioengineering college, Chongqing University,Chongqing 400044; 关键词：TOMATO; 文献传递

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国家自然科学基金(30871709)