In vitro antioxidant activities of resveratrol and piceid against peroxynitrite(ONOO-) were examined by the inhibition of 3-nitrotyrosine formation.Trolox was used as a positive control.Resveratrol and piceid exhibited high ONOO--scavenging activities in a concentration dependent manner.The antioxidant activities(the concentration of test compound required to yield a 50% inhibition of tyrosine nitration,IC 50) of resveratrol and piceid against ONOO-were(48.34±0.97) and(74.69±1.49) μmol/L,respectively.Compared with that of trolox[(105.40±1.16) μmol/L],their scavenging activities were 2.2-and 1.5-fold higher for resveratrol and piceid.Formation of nitroresveratrol as shown by UV-Vis spectroscopy and liquid chromatography-tandom mass spectrometry(LC-MS/MS) analysis indicates that resveratrol could directly scavenge ONOO-via nitration reaction.Our results demonstrate that foods and medicinal herbs with resveratrol and piceid as stronger ONOO-scavengers are valuable ingredients and have healthy application in preventing humans from peroxynitrite-mediated oxidative damage by scavenging peroxynitrite efficiently.
重组人干扰素(Recombinant human interferon,rhuIFN)是一类抗病毒、抗肿瘤和多发性硬化调节免疫的重要生物制品。自1957年发现干扰素以来,人们不断采用新技术对干扰素分子结构进行改造,先后研发了原型干扰素、保守干扰素、聚乙二醇修饰干扰素等几十个品种,目前已批准十余个一类新药干扰素上市。本文在回顾干扰素药物历史的基础上,对化学修饰改型干扰素、融合干扰素、杂合干扰素等的研究进展作一综述。
