[ Objective] The aim was to supply technical basis for developing quarantine and control techniques for orient fruit fly. [ Method] By utilizing facilities including rearing chamber and rearing cage, the population of orient fruit fly was established and sustained by using the methods of artificial feeding and fruit culture, and the continuous indoor biological observation was carried out. The isolated enclosure equipped with temperature and humidity recorder was established in Dongshan area of Taihu for cultivating various species of host plants, then the living habits of orient fruit fly were observed everyday. [ Result] Through feeding and observation in lab, the living habit of adult, larva, pupa and eggs of orient fruit fly were studied; the impact of soil depth on the emergence rate of pupa were conducted, and the result indicated that when the soil layer depth increased to 40 cm, there were still adults appeared and their emergence rate was 80%. The results of indoor trend test showed that the selectivity of orient fruit fly on fruits produced in Suzhou was sequenced from high to low as follows: tangerine, dates, pomegranate, peach, orange, pear and persimmon. While the sequence of fruit tree species were tangerine, dates, pomegranate, pears and persimmons according to damage rates of orient fruit fly from high to low under field isolation rearing conditions. In the local tangerine varieties, the damage rate from high to low were Dongting manda- rin, eady tangerine, Wen orange, Huangpi, Zaohong, Liaohong. [ Conclusion] Orient fruit fly artificial rearing facility was established, which laid the foundation for carrvina out bioloaical characteristics observation and biolooical exoeriments