目的采用气相色谱-质谱联用(GC-MS)技术对大肠风与小肠风挥发油进行对比分析,为二者合理利用提供参考依据。方法水蒸气蒸馏提取挥发油。色谱柱为HP-5MS石英毛细管色谱柱(30 m×0.25 mm,0.25μm);程序升温;载气为氦气(He),体积流量为1.0 m L/min;检测器温度为280℃;溶剂延迟为3 min;进样量为0.2μL;分流比为100∶1;空气流速为300 m L/min;尾吹气N2流速为30 m L/min。结果用水蒸气蒸馏法提取所得挥发油成分较复杂,大肠风与小肠风挥发油共有成分为石竹烯、α-石竹烯、斯巴醇、反式-橙花叔醇、α-荜澄茄油烯等14个,主要为烯烃类及醇类;大肠风特有成分为(-)-β-榄香烯、(+)-β-蛇床烯、1,5,5-三甲基-6-亚甲基-环己烯,含量都高达14%以上;小肠风中(-)-斯巴醇、反式-橙花叔醇,含量都高达15%以上。结论大肠风与小肠风挥发性成分具有差异性,可作为鉴别二者的基本依据。
[ Objective ] This study aimed to establish the infrared （IR） spectra fingerprints for peels of Citrus grandis from Guangxi Zhuang Autonomous Region. [ Method ] The fingerprint was established by fourier transform infrared spectroscopy （ FT-IR）, and then were analyzed by Omic software. [ Result ] IR spectra fin- gerprint of C. grand/s were successfully established. There were six common peaks in the characteristic spectra of 12 samples. [ Conclusion] The specific, rapid and stable method can be applied to quality control of C. grandis.
