Objective:To observe the regulation of Youguiyin(YGY,右归饮) on the gene expression profile of the rat with steroid-induced femoral head necrosis(sFHN),for the sake of investigating its molecular mechanism of sFHN prevention and treatment.Methods:All the 30 rats were randomly divided into three groups, the normal control group(A),the model control group(B),and the YGY treated group(C),10 in each group. After rats in Groups B and C were being made into FHN models with steroid injection,they received a daily intragastric administration of saline and YGY respectively in equal volume for a total of 6 weeks,while to the unmodeled normal rats in Group A,saline was administered instead.The rats were sacrificed at the terminal of administration;their mRNA from femoral head tissue was extracted and prepared to cDNA probe through inverse transcription for detecting gene expression profile by microarray,outcomes of which was passing fluorescence quantitative PCR verification,and the differential expressed genes were analyzed adopting gene ontology(GO) method.Results:Compared with Group A,the numbers of differential genes found in Groups B and C were 190 and 92,respectively,but the changing trend in the two groups was opposite,mainly manifested as down-regulating in Group B/Group A(GB/GA) and up-regulating in Group C/Group B(GC/GB).The analysis showed that these differential genes were mainly assigned to cell apoptosis,signal transduction,metabolism, cell proliferation and differentiation,cell cycle,blood coagulation,antioxidant activity,etc.Conclusions:sFHN was regulated by various genes;the regulation of YGY on expressions of these genes and the intra/extra-cellular signaling processes was possibly the molecular mechanism of YGY for preventing/treating sFHN.This study gave an explanation to the effectiveness of Chinese medicine in preventing/treating FHN from aspects of gene expression and enriched the Chinese medicine theory of "Kidney(Shen) governing bones".
[目的]研究激素性股骨头坏死(steroid-induced avascular necrosis of the femoral head,SANFH)动物模型的建立方法。[方法]将20只雄性Wistar大鼠随机分为空白对照组、模型组。采用甲基强地松龙联合大肠杆菌内毒素的方法诱导造成SANFH,对大鼠行为学观察6周后对其进行开场试验、自主活动水迷宫实验和抓力等行为学检测。[结果]与正常组相比,模型组大鼠在开场试验中的运动总距离明显减少,自主活动次数减少,在Morris水迷宫实验中平均速度减小,总活动距离延长,抓力显著降低。[结论]两组大鼠在行为学的改变,证明了甲基强地松龙联合大肠杆菌内毒素成功诱导SANFH大鼠模型,适用于SANFH的发病机制和治疗方法的进一步研究。
