The determination method of Schisandrin A and Schisandrin B in Schisandra chinensis was improved with the high performance liquid chromagraphy(HPLC).The sample was extracted exceedingly in the critical limit of CO 2 .The retention time of Schisandrin A and Schisandrin B was reduced,with methano/water(75:25)as mobile phase.The wavelength for detection was 254 nm. The R2 of standard curve was 0.9998 and the relative standard deviation was 2.31%and 3.17%with the recovery of 96.45%and 97.37%,respectively.The result shows that the rate of veracity of this method is higher and it proves that the determination method of Schisandrin A and Schisandrin B in Schisandra chinensis is a feasible method.
XU Liangmei LI Jianping YAN Changjiang SHAN Anshan
An experiment was conducted to study the response of broiler to diet dilution during the finishing period. Sixty 29-day-old commercial Avian male broiler chicks were assigned to two groups randomly, with 30 broilers each. Birds were offered a conventional finishing diet or with 10% dilution diet with ground rice hulls from 29 to 49 days of age. The result showed that there were no significant differences between the effects of diet dilution and the control on final body weight, growth rate and feed conversion efficiency. Diet dilution led to a reduction in abdominal fat weight, whereas carcass and breast muscle weight were not affected.
A 2×2×2 factorial design was adopted to study the effects of temperature,moisture and choline chloride on vitamin A stability in premix.The results indicated that temperature,moisture and choline chloride damaged vitamin A significantly.The regression equations of vitamin A disappearance rate and storage time were as follows:in room temperature(18+3)℃,y=14.368Ln(x)+ 4.1425,R^2=-0.978;in high temperature(4℃),y=22.24Ln(x)+13.27,R^2=0.9918;in low moisture(2%-3%),y=10.408Ln(x)+9.5418, R^2=-0.9322;in high moisture(8%-9%),y=26.199Ln(x)+7.8741,R^2=-0.9949;in the condition of choline chloride free,y=9.5125Ln(x)+ 8.9869,R^2=0.9826;supplemented with choline chloride,y=27.094Ln(x)+8.4276,R^2=0.9984.Temperature had highly significant interaction with moisure and choline chloride on destruction of vitamin A,respectively from the periods of two months storage. However,from the period of the first month storage,the interaction of moisture and choline chloride,as well as the interaction of temperature,moisture and choline destroyed vitamin A remarkably.
