The capability of our newly developed DNA-machine-driven strategy in tuning the association rate of DNA- AuNPs was compared with that of linker-addition strategy which has potential practical applications in different fields. The new established strategy shows its superiority to the linker-addition strategy in tuning the association rate of DNA-AuNPs in both pre-incubation treatment and non-incubation treatment conditions since its two components （a complex and a catalyst- oligonucleotide） can be individually optimized to make the machine run at an optimal rate. This strategy will provide a more convenient and flexible option in designing an oligonucleotide detection system and building a complex and versatile device.
The mechanism of the phase separation of polymer blends in the presence of a static electric field was investigated using the molecular dynamics with a modified coarse-grained polarizable model.Two electric field effects,i.e.,the promotion and hindrance of the phase separation,were found in the system of the upper critical solution temperature(UCST)by varying the dielectric properties of polymer components.Our simulation directly demonstrated that the electric field effects originate from the energy changes of intramolecular unit polarization,intermolecular van der Waals(VDW)and Coulomb interactions induced by the external electric field.The relationship of the energy change and the dielectric constant for the system under electric field is obtained.The interface orientation under electric field was found to happen even before the transition point of phase separation.By assuming that the relative order parameter of the phase separation undergoes the same fluctuations for a system with and without an electric field,the transition temperature under an electric field can be predicted,and quantitative consistency with simulations is obtained.
