A novel fluorescent probe 9-(4-(1,2-diamine)benzene-N1-phenyl)acridine(DABPA) was synthesized for the detection of nitric oxide(NO) and characterized by IR, 1H-NMR and EI-MS spectroscopy. Based on a photoelectron transfer mechanism, the fl uorescence intensities of DABPA were investigated with the different concentrations of NO. Under the optimal experimental conditions, the fl uorescence intensity of DABPA had a good linear relationship(R2=0.9977) with NO concentration in the range from 1×10-7 to 1.5×10-6 mol/L with a detection limit of 1×10-8 mol/L. The cytotoxicity induced by DABPA was evaluated by the MTT(3-(4,5-dimethylthiazol-2-yl)-2,5diphenyl tetrazolium bromide) assay for biological application. Furthermore, the probe DABPA had also been successfully applied to real-time image NO produced in PC12 cells in the presence of L-arginine.
For the surface plasmon resonance (SPR)-based optical fiber sensor with four-layer configuration (fiber core/metal/sensing layer/sample), the effects of different metals, different models (Lorentz-Drude model and Drude model) for the same metal, the thickness of metal layer, and the refractive index (RI) of sample on the transmitted spectra are investigated. The calculated results show that the real part of the refractive index of metal layer has the greatest effects on the sharp degree of the transmitted dip. Moreover, the dispersion function under four-layer configuration can be used to understand the relationship between the resonance wavelength and the thickness of metal layer.
