A novel photoelectrochemical biosensor incorporating nanosized CdS semiconductor crystals with enzyme to enhance photochemical reaction has been investigated. CdS nanoparticles were synthe-sized by using dendrimer PAMAM as inner templates. The CdS nanoparticles and glucose oxidase (GOD) were immobilized on Pt electrode via layer-by-layer (LbL) technique to fabricate a biological-inorganic hybrid system. Under ultraviolet light,the photo-effect of the CdS nanoparticles showed enhancement of the biosensor to detect glucose. Pt nanoparticles were mixed into the Nafion film to immobilize the CdS/enzyme composites and to improve the charge transfer of the hybrid. Experimental results demon-strate the desirable characteristics of this biosensing system,e.g. a sensitivity of 1.83 A/(mM cm2),lower detection limit (1 M),and acceptable reproducibility and stability.
