In this study, twelve primers were used for ISSR (inter simple sequence repeat) analysis of eight F1 hybrids of Erianthus fulvus and sugarcane (Saccharum spp.). The results showed that totally 133 bands were amplified, including 111 polymorphic bands, showing a polymorphic rate of 83.46%, which indicated that there are abundant genetic variations among F1 progenies derived from crossing between cultivated sugarcane and E. fulvus. The average genetic similarity coefficients between F1 progenies and female parent Yacheng 89-9, F1 progenies and male parent E. fulvus were 0.67 and 0.44, respectively. UPGMA clustering result of F1 progenies showed that all the progenies were clustered with female parent, suggesting that the genetic material of female parent was predominated in F1 progenies, which also revealed that the chromosome number of F1 progenies was not directly related to the clustering results based on ISSR analysis.
[Objective]This study aimed to provide a scientific basis for breeding new sugarcane varieties with hybrid progenies of Erianthus fulvus and modifying existing sugarcane cultivars.[Method]Chromosomal slides of the F2 and F2BC1hybrid progenies between sugarcane and E. fulvus( 2n = 20) and those of parental materials were prepared with the wall degradation hypotonic method,to further analyze the chromosomal transmission in the F2 and F2BC1hybrid progenies and to investigate the chromosome transmission mechanism.[Result]The results showed that,somatic chromosome number varied in various hybrid progenies; chromosomes from parents were transmitted into the somatic cells of F2 hybrids in " 2n + n" pattern and transmitted into the somatic cells of F2BC1 hybrids in " n + n" pattern.[Conclusion]This study provided a preliminary cytological basis for further use of these materials in sugarcane breeding.
Xianhong WANGFusheng LILilian HEHongbo LOUQinghui YANGShunchang HE
Chromosome specimens of sugarcane,Erianthus fulvus and their progenies were prepared with wall degradation hypotonic method to analyze the karyotypes of parents and F1,F2 hybrids. The results showed that most chromosomes of experimental materials are median region( m) chromosomes,while only a small amount of chromosomes are submedian( sm) chromosomes. The karyotype of YAU04 /102 belongs to 2C type,while other materials belong to 2B type. In addition,the karyotypes of hybrids are asymmetrical and the chromosomes of hybrids show an evolutionary trend compared with parental chromosomes.
Xianhong WANGFusheng LILilian HEHongbo LOUQinghui YANGShunchang HE
In this study,karyotype analysis of F2BC1 progenies between sugarcane( Saccharum spp.) and Erianthus fulvus was conducted. The result showed that most chromosomes of YAU04 /14,YAU09 /02,YAU09 /05,YAU09 /26 and YAU09 /52 are median region( m) chromosomes,while only a small amount of chromosomes are submedian( sm) chromosomes and median point( M) chromosomes. The karyotype formulae of five experimental materials are 2n = 106 = 98 m +8sm,2n = 106 = 2M + 104 m,2n = 106 = 4M + 96 m + 6sm,2n = 102 = 2M + 92 m + 8sm and 2n = 106 = 4M + 96 m + 6sm,respectively. The karyotypes of all materials belong to symmetrical 2B type,indicating that the chromosomes of progenies are primitive.
Hongbo LOUFusheng LIXianhong WANGLilian HEQinghui YANGShunchang HE
