In order to extract the information of 662-keV 137Cs spectra from the overlapping peaks with 583-keV 208Tl,609-keV 214Bi,the overlapping peaks are measured by in-situ γ-ray spectrometer using a NaI(Tl) detector.The spectral model is optimized by the Gaussian fitting algorithm,and the optimized fitting indexes for fitting/original value are from 0.96 to 0.99.Gaussian fitting verified by experiment is feasible for γ-ray spectrum analysis.The full energy peak of 137Cs is extracted correctly from the overlapping peaks,it is important for in-situ γ-ray spectrometer to estimate contamination of 137Cs in radiated environment and nuclear accident.