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国际竹藤网络中心基本科研业务费专项资金项目(1632013008)

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相关作者:彭镇华胡陶姚娜杨学文李潞滨更多>>
相关机构:赤峰市农牧科学研究院中国林业科学研究院国际竹藤中心更多>>
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Construction and Quality Analysis of Full-length cDNA Library of Phyllostachys heterocycla Germinating Seeds
2013年
[Objective] This study aimed to construct the full-length cDNA library for ger- minating seeds of Phyllostachys heterocycla [Method] Germinating seeds of P. hetero- cycla were used as experimental materials to construct the full-length cDNA library by using Oligo-capping method. [Result] The constructed library has a total capacity of 6.5×10^6 recombinant clones, and a low proportion of clones without inserted frag- ments; the size of inserted fragments ranges between 0.3-5.0 kb, with strict classifi- cation and ideal consistency. Furthermore, the proportion of clones harboring long in- serted fragments (1.0-5.0 kb) is as high as 30%, achieving the standard for high- quality full-length cDNA library. [Conclusion] The full-length cDNA library of germinat- ing seeds of P. heterocycla was successfully constructed, which laid important foun- dation for the functional genomics research of bamboo plants.
胡陶姚娜杨学文彭镇华李潞滨
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