Schistosoma japonicum Katsurade is a major threat to the heath of Chinese people and prevent social economies ...
Wan-xian WANG,Yi YANG,Yong ZHANG,Qian CHEN,Xu LIU,Xiu-li XIANG,Hui-dong JIANG,Wen CHEN and Wei-lin YUN Institute of Biology and Science,Hubei University,Wuhan 430062,China
The expression vector of anti Hantavirus(HV) capsid protein scFv was constructed by DNA recombination and PCR technique,and expressed in E.coli . The expressed scFv was fused with the phage GeneⅢ protein, and located on the surface of phage.The sequencing of scFv demonstrated that the sequence of scFv is consistent with that of cloned antibody variable region from F3 strain hybridom against HV capsid protein.The expressed scFv was proved to be able to bind HV antigen by ELISA.
