Objective: To study the effects of angiostatin (AS)gene mediated by liposome on human pancreaticcancer cell line SW1990.Methods: Angiostatin gene was cloned into the eu-karyotic expression vector pRC/CMV. The recombi-nant of pRC/CMV-AS was introduced into the pan-creatic cancer cell line, SW1990. The mechanism ofanti-tumor was studied and tested.Results: The eukaryotic expression vector pRC/CMV-AS was identified by the restriction digest.pRC/CMV-AS was stably integrated into the targetcells and expressed by Western blot and drug-sensi-tivity tests, and inhibited the vascular endothelialcells proliferation in vitro. In addition, the effects ofthe angiostatin vector on reducing the volume oftumors implanted in nude mouse models were alsonoted.Conclusion: This study demonstrated that the recom-binant pRC/CMV-AS mediated by liposome mayplay a potential role in the treatment of pancreaticcancer in the future.
