OBJECTIVE:To establish a quantification model of Traditional Chinese Medicine(TCM)syndromes by sampling patients undergoing idiopathic precocious puberty(IPP)and early puberty.METHODS:A questionnaire for classifying and quantifying TCM syndromes was designed and administered.All the results were analyzed;the relationship between 3 types of syndrome and 47symptoms were summated.Meanwhile,the frequency distribution of each symptom or sign was aggregated.Fuzzy mathematics was used to develop a quantification model ofTCM syndromes.RESULTS:We found that precocious puberty had 3types of syndrome,including hyperactivity of fire due to Yin deficiency(Syndrome I),depressed liver Qi transforming into fire(Syndrome II),and end retention of damp heat(Syndrome III).In the IPP group,Syndrome I was the most common principal syndrome(100%).Forty-six patients(43.81%)werediagnosed with Syndrome I accompanied by Syndrome II and 11(10.48%)were diagnosed with Syndrome I accompanied by Syndrome III.In the early puberty group,Syndrome I was also the main syndrome(98.39%).The degrees of most symptoms were mild to moderate.Reddened tongue was the most common tongue manifestation(62.86%prevalence)in the IPP group.The most common pulse manifestations were slippery pulse,thread pulse,and taut pulse.The Asymptotic Normalization Coefficient(ANC)method was used to quantify the TCM syndromes in 167 cases.Diagnostic accuracy rate reached 91%,comparable to expert diagnosis.CONCLUSION:We find that there are 3 types of syndrome in the IPP group and in the early puberty group.Syndrome I(hyperactivity of fire due to Yin deficiency)is the main syndrome in the two groups.ANC may be an appropriate for quantification model ofTCM syndromes.
Yanyan LinYuanyuan XueJian YuYonghong WangWen SunYanyan SunYumin ShiYiqun ZhangHong Hu
目的:观察光照改变对雄性金黄地鼠下丘脑-垂体-性腺轴的影响。方法:2日龄雄性金黄地鼠40只,随机分为正常组(12h,50lux)、16h弱光组(16h,50lux)、16h强光组(16h,350lux)和20h强光组(20h,350lux),光照干预至28日龄;放免法检测血清睾酮(T)、黄体生成素(LH)、促卵泡激素(FSH)水平;Real-time PCR法检测下丘脑促性腺激素释放激素(Gn RH)、促性腺激素抑制激素(Gn IH)、褪黑素受体1c亚型(Mel1c),垂体Gn RHR、GPR147 m RNA表达变化。结果:光照组金黄地鼠出现躁动不安,皮毛光泽度差,体质量偏轻等类似阴虚火旺证候表现。与正常组比较,各光照组T水平显著上升(P<0.05);下丘脑Mel1c m RNA表达水平显著下降(P<0.01,P<0.05);16h弱光组和20h强光组下丘脑Gn IH m RNA表达水平显著升高(P<0.05)。结论:延长光照时间、增加光照强度可使雄性地鼠出现一定的阴虚火旺表现。光照时间延长、强度增加可引起雄性地鼠睾丸发育提前,其机制可能与褪黑素受体、Gn IH有关。
