[Objective] To observe biological characteristics of brown-egg dwarf layers. [Method] Physiological and biochemical indicators of brown-egg dwarf layers were determined at the age of 20, 40 and 60 weeks, respectively. Fifty-four chickens were detected, nine roosters and nine hens at each age. [Result] As analyzed by t test and multiple comparisons, during 20-60 weeks old, the RBC, HGB, MPV, PDW, LYMPH%, TP and CHO showed an increasing trend. However, during this period, the HCT, MCV, RDW, WBC and LYMPH first decreased and then declined, while the MCH, MCHC, PLT, PCT, MID, MID%, GRAN, GRAN%, AST, ALB, TG, ALT and GLU changed contrarily. The chickens at different ages had significantly different physiological and biochemical indicators (P < 0.05). The RBC, HCT, HGB, WBC, LYMPH and AST were significantly different between the roosters and hens during 20-60 weeks old. [Conclusion] In the brown-egg dwarf layers, the physiological and biochemical indicators vary with aging, and their difference levels also change with aging.