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国家自然科学基金(30671048)

作品数:4 被引量:14H指数:2
相关作者:陈长军王建新周明国金丽华陈雨更多>>
相关机构:南京农业大学西南大学更多>>
发文基金:国家自然科学基金国家重点基础研究发展计划国家高技术研究发展计划更多>>
相关领域:农业科学生物学更多>>

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抗氰烯菌酯的禾谷镰刀菌nit突变体的诱导及其生物学特性被引量:7
2007年
【目的】研究抗氰烯菌酯的禾谷镰刀菌nit突变体的生物学特性。【方法】将3个抗氰烯菌酯的禾谷镰刀菌(Fusarium graminearum Schw.)菌株分别在含氯酸盐的MMC培养基上培养,共获得了50个硝酸盐利用缺陷突变体(nit)。比较了抗性菌株的nit突变体与亲本在无性和有性阶段的主要生物学性状。【结果】抗性菌株的nit突变体与亲本在菌落生长速率、培养性状和致病性方面没有显著差异;但某些突变体中,产孢量和产子囊壳能力方面存在一定差异。此外,禾谷镰刀菌对氯酸盐和氰烯菌酯之间没有交互抗药性,且抗性可以稳定遗传。【结论】可以将nit作为遗传标记来研究禾谷镰刀菌对氰烯菌酯的抗药性遗传学。
陈雨陈长军王建新金丽华周明国
关键词:禾谷镰刀菌生物学性状
禾谷镰孢菌两个β-微管蛋白基因功能
本文分别敲除禾谷镰孢菌的β1、β2-微管蛋白基因,并测定各敲除突变体菌株对多菌灵的敏感性,结果表明,敲除其中任意一个β-微管蛋白基因的突变体菌株都对多菌灵敏感,但敏感性有差异:与野生菌株相比(EC50=0.56μg/ml...
毕朝位周明国陈长军王建新
关键词:小麦赤霉病禾谷镰孢菌Β-微管蛋白基因功能
多菌灵对禾谷镰孢菌分生孢子萌发和有丝分裂的影响
禾谷镰孢菌Fusarium graminearum Schwabe是我国引起小麦赤霉病的主要病原菌,多菌灵是防治该病害的主要药剂之一。已有研究结果表明多菌灵对灰葡萄孢菌Botrytis cinerea
毕朝位仇剑波周明国陈长军王建新
关键词:禾谷镰孢菌灰葡萄孢菌有丝分裂多菌灵抗药性
Activity of Azoxystrobin and SHAM to Four Phytopathogens被引量:2
2009年
The study was conducted to make clear the activity of azoxystrobin to 4 plant pathogens and the synergistic effects of salicylhydroxamic acid (SHAM), which acted on the alternative oxidase. It was also conducted to be aware of the mechanism of azoxystrobin in inhibition on mycelial respiration and the influence of SHAM. The activity test of azoxystrobin and SHAM was carried out with a mycelial linear growth test and spore germination test. Other related biological properties were also observed. Inhibition of azoxystrobin and SHAM on 4 pathogens was determined by using SP-II oxygraph system. Azoxystrobin inhibited mycelial growth in Colletotrichum capsici, Botrytis cinerea, Rhizoctonia solani, and Magnaporthe grisea, respectively; it also inhibited conidia germination, and conidia production in C. capsici, B. cinerea M. grisea, and sclerotia formation in R. solani. Moreover, it created stayed pigment biosynthesis in C. capsici and M. grisea somehow. Salicylhydroxamic acid enhanced inhibition by azoxystrobin. An oxygen consuming test of the mycelia showed that azoxystrobin inhibited all the 4 fungi's respiration in the early stages. With the concentration rising up, the effectiveness increased. However, as time went on, the respiration of the mycelia treated with fungicides recovered and SHAM could not inhibit the oxygen consuming. This reaction between the mycelia and the fungicides appeared not to initiate alternative respiration but rather the other mechanism created a lack of efficacy.
JIN Li-hua CHEN Yu CHEN Chang-jun WANG Jian-xin ZHOU Ming-guo
禾谷镰孢菌Fusarium graminearum Schwabe分生孢子萌发过程中的核相变化及有丝分裂过程观察被引量:3
2008年
通过Giemsa染色观察禾谷镰孢菌Fusarium graminearum分生孢子萌发过程中的核相变化及有丝分裂过程。观察表明,分生孢子细胞为单核,细胞核在分生孢子细胞内分裂后进入芽管,在芽管内进行多次分裂,使芽管内细胞核数目不断变化。禾谷镰孢菌有丝分裂过程可以分为4个时期,前期染色体逐渐浓缩变短,中期染色体清晰可见,后期染色单体发生分离并向相反的两极移动,末期形成新的子核。有丝分裂过程中染色体的分离同步或不同步,不同步分离中的滞后染色体形成后期桥的现象更为普遍。
毕朝位仇剑波周明国王建新陈长军李红霞
关键词:GIEMSA染色细胞核
Effect of Azoxystrobin on Oxygen Consumption and cyt b Gene Expression of Colletotrichum capsici from Chilli Fruits被引量:2
2009年
Azoxystrobin acts as an inhibitor of electron transport by binding to the Qo center of cytochrome b (cyt b). Resistance to azoxystrobin was usually caused by the point mutation of cyt b gene or by the induction of alternative respiration. Oxygen consumption test for mycelia of Colletotrichum capsici showed that azoxystrobin inhibited mycelial respiration within 12 h; however, as time went on, the respiration of the mycelia recovered when the mycelia were treated with azoxystrobin and salicylhydroxamic acid (SHAM, a known inhibitor of alternative respiration), and the oxygen consumption of the mycelia could not be inhibited. Meanwhile, cytochrome b (cyt b) gene expression increased with the recovery of mycelial respiration. The increased cyt b gene expression might play a role in the development of resistance to azoxystrobin in C. capsici.
CHEN Yu JIN Li-hua ZHOU Ming-guo
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