A novel pH-sensitive nanoparticle drug delivery system (DDS) derived fl'om natural polysaccharide pullulan for doxorubicin (DOX) release was prepared.Pullulan was functionalized by successive carboxymethylization and amidation to introduce hydrazide groups.DOX was then grafted onto pullulan backbone through the pH-sensitive hydrazone bond to form a pullulan/DOX conjugate.This conjugate self-assembled to form nano-sized particles in aqueous solution as a result of the hydrophobic interaction of the DOX.Tr...
Three cationic lipids with lysylated(1),histidylated(2),and arginylated(3) headgroups and cholesterol hy-drophobic moiety were synthesized.The average sizes of liposomes and lipoplexes were around 100 and 160 nm,re-spectively.The gene transfection efficiency of the three lipoplexes loaded with pGL3 or pORF-LacZ was compared on 293T cells in the presence or the absence of serum.The transfection efficiency of the three lipoplexes in a se-rum-free medium was 2 to 3-fold higher than that of dioleoyl-trimethylammonium propane(DOTAP).In the presence of serum,however,most of the lipoplexes showed lower transfection activities;only lipoplex 3 retained its high transfection efficiency.
A six-member cyclic carbonate with protected functional groups was synthesized from tetramethylolmethane,benzaldehyde and ethyl choroformate.Coplymerization of（D,L-lactide） with cyclic carbonate was carried out through conventional ring-opening polymerization using Sn（Oct）2 as the catalyst.The effects of different catalysts,polymerization time and monomer-to-catalyst molar ratio on the molecular weight were investigated.The methods of removing protected groups were studied.Results showed the protected groups were effectively removed via catalytic hydrogenation.（Poly（D,L-lactide-co-cycliccarbonate）s） with hydroxyl pendant functional groups was obtained.
