Leptobrachium bompu Sondhi and Ohler, 2011 was described based on a single specimen collected from Eaglenest in southern slope of Himalaya(holotype: No. KA0001/200905). In April, 2014, seven adults and three tadpoles of Leptobrachium bompu were collected from upper Medog, Tibet, China since most the morphological characters of the adult frogs matched the species Leptobrachium bompu, while the tadpoles mentioned above were determined as the same species as the adults by molecular systematics analysis. Three types of iris coloration were found in the seven adult specimens and two types of spiraculum in the three tadpoles. Advertisement calls usually had 6-8 notes and the fundamental frequency ranged from 1076 to 1466 Hz. All the specimens collected at upper Medog were clustered as one lineage with very low genetic variation and located at the base of the phylogenetic tree of the genus Leptobrachium.
We described a new species, Microhyla beilunensis sp. nov., from Zhejiang Province of China. Phylogenetic analyses based on the mitochondrial 12 S, 16 S and CO1 gene sequences suggested that the new taxon was distinctly separated from its congeners and closed to M. mixtura and M. okinavensis. Morphologically, the new species could be identified from its congeners except M. mixtura by several characters:(1) rudimentary webs on toe base;(2) absence of disks and dorsal median longitudinal grooves on finger tips;(3) presence of disks and dorsal median longitudinal grooves on toe tips. As well, the new species could be identified from topotype M. mixtura by the combination of characters:(1) apart from the stripes, bar-shaped and oval-shaped patterns, the rounded spots present on the dorsum of body and legs;(2) the outer metacarpal tubercles prominently larger than the inner one;(3) of males, the ratios of HW, IND, UEW and LAW to SVL of the new species were significantly larger than those of M. mixtura(P < 0.01), and the ratios of SL, IOD, LAHL, HLL, TL, TFL and FL to SVL of the new species were significantly less than those of M. mixtura(P < 0.05).
