随着生命科学的迅速发展,细胞工程愈来愈受到人们的重视。以昆虫细胞为对象的细胞培养技术在现代实验生物学上具有重要的价值,已经广泛地应用于医学、农业及生物学的各个领域。本文综述了有关昆虫细胞培养的研究进展,包括昆虫细胞培养基研究开发,昆虫细胞系的建立和组织培养,利用生物反应器大规模培养昆虫细胞,昆虫细胞-杆状病毒表达系统(B acu lov irus express ion vector system,BEV S),构建基因工程细胞系及其稳定性表达,以及昆虫细胞培养的应用前景和研究展望。
A transformed cell line was constructed fromMythimna separata cells Ms7311 by lipofection methodTMs7311 cells were generated using a double selection tech-nique involving a selection in the antibiotic Zeocin, followedby a second round of selection by exhibiting cell characteri-zation. A cell clone expressing p35 was obtained with highlevel of AcMNPV and recombinant proteins. Compared withwild type Ms7311 cells, the cell clone showed increased resis-tance to Actinamycin D-induced apoptosis and a profoundresistance to nutrient development (PBS). When the cellclone was infected with recombinant baculoviruses express-ing secreted alkaline phosphatase (SEAP) and β-galactosi-dase, expression of the recombinant proteins from TMs7311cells exceeded that from parental Ms7311 cells. Production ofbudded virus and occlusion body was significantly higherthan that from parental cells Ms7311.
ZHENG GullingLI ChangyouLI GuoxunWANG PingRobert R. Granados
