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相关作者:熊先军方智远杨丽梅刘明月更多>>
相关机构:中国农业科学院蔬菜花卉研究所湖南农业大学更多>>
发文基金:国家自然科学基金更多>>
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蔬菜转基因研究进展被引量:3
2004年
熊先军杨丽梅刘明月方智远
关键词:蔬菜转基因抗虫基因
农杆菌介导的Bt基因crylAh转化结球甘蓝的研究
以结球甘蓝(Brassica oleracea var.capitata)的下胚轴和具柄子叶为外植体,通过根癌农杆菌(Agrobacterium tume faciens)介导法将苏云金芽孢杆菌(Bacillus thu...
崔磊杨丽梅方智远刘玉梅庄木张扬勇孙培田
关键词:根癌农杆菌BT基因结球甘蓝
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Expression in Arabidopsis of a Strawberry Linalool Synthase Gene Under the Control of the Inducible Potato PI2 Promoter被引量:1
2008年
To investigate the role of inducible linalool in Arabidopsis-insect interactions, the FANES 1 linalool synthase (LIS) cDNA from strawberry with plastid targeting and a synthetic intron (LIS') was placed under the control of the wound inducible proteinase inhibitor 2 (PI2) promoter from potato. The construct pBin-PPi2-LIS' was transformed to Arabidopsis thaliana ecotype Columbia 0. Kanamycin resistant TO seedlings were confirmed for the presence and transcription of the LIS' gene by PCR analysis on genomic DNA and by RT-PCR analysis on RNA. Genomic and RT-PCR products were sequenced to confirm correct splicing of the synthetic intron. The expression of active linalool synthase by the PPI2-LIS' gene construct in the transgenic lines was assessed by measuring linalool emission using solid phase micro-extraction (SPME) GC-MS measurements after induction with methyl jasmonate. Among 30 tested independent T2 transgenic lines, 10 exhibited linalool production. Linalool expression could be induced by methyl jasmonate treatment, but not by diamondback moth larvae.
YANG Li-meiPer MerckeJoop J A van LoonFANG Zhi-yuanMarcel DickeMaarten A Jongsma
关键词:EXPRESSION
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