The tannins immobilized chitosan-Ce4+ resins(TCCR) were prepared. The optimum conditions on activating and coupling of chitosan resins were obtained by means of orthogonal design experiments. TCCR were characterized by DSC and FT-IR. The adsorption capacity for bovine serum albumin(BSA) of TCCR was investigated. The results show that TCCR have an excellent adsorption capacity for BSA in water,comparing with chitosan-Ce4+ resins(CCR) . The TCCR can be a novel functional material for removal protein in some beverages.
Bentonite was modified with chitosan and REIs(La3+,Ce3+) to form chitosan-RE3+-bentonite(CRB) . CRB were characterized by DSC and FT-IR. And the inhibitory effects of CRB on the growth of Chlorella vulgaris were investigated. The results indicated that CRB could inhibit the growth of Chlorella vulgaris. The relative conductivity and the generation of malondialdehyde(MDA) increased. In addition,the superoxide dismutase(SOD) and peroxidase(POD) activities decreased. So CRB could be a promising algaecide agent.
In this study, the water-based ferromagnetic fluid and magnetic resin made from chitosan and cerium complex （MRCCC） were successfully prepared by using the chemical co-precipitation technique and by the reversed-phase suspension cross-linking polymerization. MRCCC presented uniform and narrow panicle size distribution as determined by the Laser Panicles Sizer. The Inductively Coupled Plasma-Atomic Emission Spectrometry （ICP-AES）, Fourier transform infrared spectroscopy （FT-IR）, differential scanning calorimetry （DSC） and X-ray powder diffraction （XRD） study demonstrated that there were iron and cerium existing in MRCCC. The movement of MRCCC under magnetic field proved its magnetic property. The swelling kinetics in water or solutions with different pH indicated that MRCCC could be applied in solutions with pH greater than 1.0. The ferromagnetic fluid particles were stable in MRCCC soaked in solutions with pH 〉2.0. In view of these results, MRCCC can be used as material for separation, clarification, adsorption, sustained release and hydrolysis activity.
以基础饲料中添加500mg/kg壳寡糖(Chitosan oligosaccharide,COS)喂养初始体质量为(12.2±0.01)g的大菱鲆(Scophthalmus maximus L.)8周,研究COS对大菱鲆生长、血清免疫相关酶活性和抵抗迟缓爱德华氏菌感染能力的影响。结果表明:饲料中添加壳寡糖后,大菱鲆成活率得到提高,特定生长率和增重率分别提高了15.79%和25.26%。相比对照组,饲料中添加壳寡糖饲养的大菱鲆血液中性粒细胞的吞噬指数提高了4.51%,酸性磷酸酶活性提高了32.89%,同时,大菱鲆抵抗迟缓爱德华氏菌感染的免疫保护力显著增强(P<0.05)。研究表明,在饲料中添加壳寡糖能够提高大菱鲆的生长、非特异性免疫功能和免疫保护力。
This study investigated the effect of a chitosan oligosaccharide-Ca complex (COS-Ca) on the depuration of cadmium (Cd) from Chlamys ferrari. After exposure to 0.5 mg L-1 CdCl2 for 3 or 7 d, the scallops were treated by COS-Ca prior to determina-tion of Cd, calcium (Ca) and zinc (Zn) contents, Cd distribution in organs, malondialdehyde (MDA) content and antioxidant variables. Results showed that COS-Ca reduced Cd content in the viscera of the scallops, with highest Cd depuration rate (47%) observed on day 3. The COS-Ca concentration substantially affected Cd depuration, and the exposure to 8.75 mg L-1 COS-Ca led to significantly higher Cd depuration rate compared with those of lower COS-Ca concentrations (1.75, 3.5, 5.25, and 7.00 mg L-1). Distribution analysis of Cd in scallop organs indicated that COS-Ca significantly reduced Cd content in the kidney throughout the 5-d experiment, as well as in the gill during the early stage of Cd depuration. In addition, COS-Ca treatment decreased glutathione peroxidase (GSH-Px) activity and MDA content while increasing superoxide dismutase (SOD) and catalase (CAT) activities on different days. Our work suggested COS-Ca complex treatment as an effective method for acceleration of Cd depuration from Cd-contaminated bivalves.
