[Objective] This study aimed to explore the relationship between NAC transcription factor and resistance to low temperature in tomato. [Method] Tomato seedlings were used as experimental materials to clone the N-terminal conserved domain of NAC1 gene with RT-PCR and investigate the expression patterns of NACl gene under 4 ℃ with real-time quantitative PCR （RT-qPCR）. [Result] NACl gene was expressed in roots, stems and leaves of tomato, but the expression level varied with the change of treatment duration. Moreover, the expression levels of NACl varied among different organs of tomato. The expression level of NACl in roots was much higher than that in stems and leaves. [Conclusion] This study laid a theoretical foundation for further investigation of the biological function of NAC1 gene in tomato.
