Clausena lansium, Citrus sinensis and Poncirus trifoliata belong to the different genus of Rutaceae. On the basis of LEAFY (LFY in brief) gene sequences of C. sinensis and P. trifoliata, a pair of 21 bp primers was designed and used to amplify about a 2 300 bp DNA fragment from C. lansium by PCR. The DNA fragment was cloned into pMD18-T vector and then sequenced. The sequence analysis showed that the genomic DNA clone with 2 346 bp contained a complete coding sequence of LFY homologous gene (ClLFY) which 3 exons were composed of a completed open reading frame and encoded 397 amino acids. The identities of nucleotide sequences of LFY genes between C. lansium with C. sinensis and P. trifoliata were more than 92%, and their identities of amino acid sequences were 95%.The result also indicated that C. lansium LFY gene was one amino acid less than the LFY homologous genes from C. sinensis and P. trifoliata.The research laid a sound foundation for studying the regulation and control in flowering mechanism in C. lansium at the molecular level in the future.
[目的]研究杨梅(Myrica rubraSieb et Zucc.)组织培养过程中防污染和防褐化问题。[方法]以东魁杨梅品种茎段作为离体培养材料,研究不同消毒灭菌方法、不同茎段部位和不同防褐化措施对外植体离体培养的影响。[结果]以75%乙醇1min+0.1%HgCl27min对外植体的灭菌效果最好,污染率为30.00%,褐化枯死率为30.00%,茎段诱导成功率为40.00%;在3个茎段部位中,以半木质化茎段诱导效果最好,污染率为30.00%,褐化枯死率为33.33%,诱导成功率为36.67%;在培养基中添加活性炭1.5g/L+聚乙烯吡咯烷酮(PVP)2.5g/L对外植体防褐化效果最好,褐化率由对照的60.93%下降到21.88%。[结论]该研究可为杨梅优良品种的扩繁推广和高效遗传转化体系的建立奠定良好的基础。
