The aim of this study is to evaluate the efficacy of total saponins of Dioscorea(TSD), an extract of the Chinese herbal Bi Xie, on hyperuricemia and to elucidate the underlying mechanisms. The rat hyperuricemia model was established by administration of adenine. Thirty-two rats were randomly allocated into 4 groups: model group, low/high-dose TSD-treated groups, and allopurinol-treated group. Meanwhile, 8 rats were used as normal controls. Serum uric acid(UA), blood urea nitrogen(BUN), serum creatinine(Scr), and organic anion transporting polypeptide 1A1(OATP1A1) levels were measured. Comparison between the model group and treatment(allopurinol and TSD) groups showed the serum UA levels were significantly decreased in treatment groups. TSD had similar effects to allopurinol. It was found that the OATP1A1 protein expression levels in treatment groups were higher than in model group and normal controls. And different from the allopurinol-treated groups, TSD-treated group had elevated OATP1A1 expression levels in the stomach, liver, small intestine and large intestine tissues. It was suggested that TSD may facilitate the excretion of UA and lower UA levels by up-regulating OATP1A1 expression.
目的:分析脾虚肝癌小鼠有机阴离子转运肽2a1(Oatp2a1)m RNA和蛋白的表达情况,探讨脾虚因素对肝癌的影响及其机制。方法:60只小鼠分成正常对照组、原位移植肝癌组和脾虚原位移植肝癌组,建立脾虚原位移植肝癌小鼠模型,测量各组肝癌肿瘤的体积,采用RT-PCR和Western Blot的方法检测小鼠各组织中Oatp2a1的表达。结果:脾虚原位移植肝癌组小鼠肝癌肿瘤体积明显大于原位移植肝癌组(P<0.01),肝癌造模后小鼠各组织Oatp2a1的表达发生了变化,癌组织中Oatp2a1表达均明显高于相应组肝组织(P<0.01),与原位移植肝癌组小鼠比较,脾虚原位移植肝癌组小鼠胃、肝和脾组织Oatp2a1 m RNA表达明显降低(P<0.01),癌组织表达则明显升高(P<0.01)。结论:脾虚因素能促进小鼠肝癌的发生和发展,其机制可能是脾虚内环境下各组织Oatp2a1的异常表达,进一步提示脾虚湿浊转运障碍与肝癌密切相关。
