Estrone has been identified as a potential endocrine-disrupting chemical(EDC)[1].Estrone is usually quantified by gas chromatography-mass spectrometry(GC-MS),GC-MS/MS,high performance liquid chromatography(HPLC),HPLCMS,and HPLC-MS/MS,etc.[2-3].Meanwhile,several immunoassays based on radioimmunoassay,enzyme linked immunosorbent assay(ELISA)or chemiluminescence immunoassay(CLIA)for determination of estrone in real samples have been developed[2,4].Although these methods are sensitive,they need multistage separation and are thus time-consuming and laborious.A very promising way for the simplification of immunoassays for routine applications is a shift from heterogeneous methods(with separation)to homogeneous assays(without separation)[5].
ZHANG XuanWANG QiangYU ZhongSergei A. EreminYU Chun FaiLIU JinSUN Yuan MingLEI Hong Tao
