Three new diterpenoids,including two halimanes,5(10),13E-halimadiene-3α,15-diol(1),and 5(10),14-halimadiene-3α,13ξ-diol(2),one labdane,12-(3-methyl-furan)-labd-8(17)-en-19-oic acid(3),together with sixteen known compounds were isolated from the barks of Dysoxylum densiflorum.All compounds were elucidated by extensive spectroscopic analysis.
Ji GUSheng-Yan QIANGui-Guang CHENGYan LIYa-Ping LIUXiao-Dong LUO
目的:研究尖山橙和龙州山橙中的抗肿瘤生物碱成分方法:采用层析柱和制备高效液相进行化合物分离,运用1D、2D-NMR及MS等波谱分析技术鉴定化合物结构。采用MTT法进行人体肿瘤细胞毒体外活性实验。结果:分离得到两个新的双吲哚生物碱melofusine I (1)和melomorsine I (2),以及22个已知吲哚生物碱,测试了24个化合物对5种(SW480,SMMC-7721,HL-60,MCF-7,A-549)人体肿瘤细胞的细胞毒活性。结论:化合物1-2是新的双吲哚生物碱,化合物tabersonine(3) and 19S-vindolinine(14)具有抑制5种人体肿瘤细胞株的活性。
