目的比较青春发育期体重正常和肥胖儿童青少年身体成分、非脂肪组织指数（fat-free mass index，FFMI）和脂肪组织指数（fat mass index，FMI）及其随年龄变化趋势和性别差异。方法选择7～15岁体重正常和肥胖儿童356名，每组各性别、年龄约10名，采用DEXA法测定身体成分，根据公式FFMI（kg·m^-2）=非脂肪组织（FFM）（kg）／身高（m）^2，FMI（kg·m^-2）=脂肪组织（FM）（kg）／身高（m）^2计算FFMI和FMI。结果各年龄组男、女生肥胖儿童身体成分中脂肪组织和去脂肪组织的实测值均高于体重正常儿童（P〈0．05）。7～15岁肥胖儿童FFMI和FMI均随年龄增长而增加，并高于同性别、同年龄组体重正常儿童，差异有统计学意义（P〈0．05）。结论肥胖儿童脂肪组织、去脂肪组织含量、FFMI、FMI，与体重正常儿童存在差异，男女间差异明显；FFMI和FMI是肥胖儿童的体重管理中值得应用的监测指标。
Background KCNJ11, ABCC8, PPARG, and HNF4A have been found to be associated with type 2 diabetes in populations with different genetic backgrounds. The aim of this study was to test, in a Chinese Han population from Beijing, whether the genetic variants in these four genes were associated with genetic predisposition to type 2 diabetes. Methods We studied the association of four representative SNPs in KCNJ11, ABCC8, PPARG, and HNF4A by genotyping them using ABI SNaPshot Multiplex System in 400 unrelated type 2 diabetic patients and 400 unrelated normoglycaemic subjects. Results rs5219（E23K） in KCNJ11 was associated with genetic susceptibility to type 2 diabetes （OR=1.400 with 95% CI 1.117 1.755, P=0.004 under an additive model, 0R=1.652 with 95% CI 1.086 2.513, P=0.019 under a recessive model, and OR=1.521 with 95% Cl 1.089 2.123, P=0.014 under a dominant model） after adjusting for sex and body mass index （BMI）. We did not find evidence of association for ABCC8 rs1799854, PPARG rs1801282 （Pro12Ala） and HNF4A rs2144908. Genotype-phenotype correlation analysis revealed that rs1799854 in ABCC8 was associated with 2-hour postprandial insulin secretion （P=0.005） after adjusting for sex, age and BMI. Although no interactions between the four variants on the risk of type 2 diabetes were detected, the multiplicative interaction between PPARG Pro12Ala and HNF4A rs2144908 was found to be associated with 2-hour postprandial insulin （P=-0.004 under an additive model for rs2144908; and P=0.001 under a dominant model for rs2144908） after adjusting for age, sex and BMI, assuming a dominant model for PPARG Pro12Ala. Conclusions Our study replicated the association of rs5219 in KCNJ11 with type 2 diabetes in Chinese Han population in Beijing. And we also observed that ABCC8 as well as the interaction between PPARG and HNF4A may contribute to post-challenge insulin secretion.
WANG Fang HAN Xue-yao REN Qian ZHANG Xiu-ying HAN Ling-chuan LUO Ying-ying ZHOU Xiang-hai JI Li-nong
