The immune responses of plants to foreign pathogens have developed relevant defense mechanisms, which formed complicated disease resistant signal transduction pathways. Salicylic acid（SA）, jasmonic acid（JA）/Ethylene（ET） and brassi- nosteroid （BR） could trigger the immune response to different pathogens in plants, making the plants show some resistance to the pathogens. The study on the trans- duction pathways of these three disease-resistant signals were introduced to provide some useful suggestions for the study on the transduction of disease-resistant sig- nals in plants.
As members of a super gene family, WRKY transcription factors are widely distributed in higher plants. ln this study, bioinformatic analysis of WRKY35, a member of the WRKY gene family, was carried out. Results indicated that tran-scription factor WRKY35 harbors a WRKYGQK core domain and a Cys2His2 or Cys2His/Cys zinc finger in the 5’ end without transmembrane domain. After PCR amplification and restriction digestion, WRKY35 gene fragment was ligated to prokaryotic expression vector PET28. This study provided basis for expression anal-ysis of WRKY35 protein and subsequent functional identification of WRKY35 gene.
