Segregation distortion of molecular markers has been reported in a broad range of organisms. It has been detected in an interspecific BC1 Populus pedigree established by controlled crossing between clone ＂LM50＂ （Populus tomentosa） and its hybrid clone ＂TB01＂ （P. tomentosa × p. bolleana）. The study with a total of 150 AFLP markers （approximately 18.9% of the total loci） exhibited significant deviation from the Mendelian ratio （1：1） （p〈0.01）. Twenty-five percent of the markers were mapped on the parental specific genetic linkage maps of clones ＂LM50＂ and ＂TB01＂ with a pseudo-test-cross mapping strategy. Twelve linkage groups had markers with skewed segregation ratios, but the major regions were on linkage groups TLG2, TLG4 and TLG6 in the linkage map of clone ＂LM50＂. We also analyzed the association between distorted loci and expression of complex traits with Mapmaker/QTL software. A total of 16 putative QTLs affecting 12 traits were identified in the distorted regions on seven linkage groups. Therefore we could detect the distribution of skewed loci along the entire genome and identify the association between quantitative traits and segregation loci via genetic mapping in an interspecific BC1 P. tomentosa family. Furthermore, the genetic nature and possible causes of these segregation distortions for differentiation between female and male parents were also discussed.
Experiments were carried out by introspecific controlled crossing between a female Populus tomentosa Carr.(clone 5082) and male P.tomentosa Carr. var. truncata Y. C. Fu et C. H. Wang, var. nov. 3?193 seeds were obtained and then dibbled on 1/2 MS medium in vitro, and cultured by 25℃ light shining in 15 days. The rate of germination was 48.74%. Among them, there were 1?179 root seedlings and 376 non root seedlings. The segregation ratio of offspring (rooting∶non rooting ) was 3∶1 in Populus tomentosa and χ 2=0 557< χ 0 05 =3.84. The conclusion is that the rooting character is belonged to qualitative trait and controlled by a single complete dominant locus.
