A new lipophilic monosaccharide, erigearide A （1）, was isolated from the aerial parts of Erigeron annuus （Lima.） Pers. Its structure was elucidated by analysis of spectroscopic evidence.
＜正＞Paclitaxel represents a potent and effective class of anticancer compounds by causing mitotic arrest and mi...
Han-Zhang XU~1,Ying HUANG~(1,2),Ying-Li WU~1,Yong ZHAO~3,Wei-Lie XIAO~3,Yixin Yao~2, Han-Dong SUN~3,Wei DAI~2,and Guo-Qiang CHEN~(1*) 1 Department of Pathophysiology,Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education,Shanghai Jiao-Tong University School of Medicine,Shanghai 200025,CHINA. 2 Department of Environmental Medicine and Pharmacology,New York University School of Medicine, Tuxedo,New York 10987,United States. 3 State Key Laboratory of Phytochemistry and Plant Resources in West China,Kunming Institute of Botany,Chinese Academy of Sciences,Yunnan,CHINA.
OBJECTIVE Eriocalyxin B(EriB)is a natural diterpenoid purified fromIsodoneriocalyxvar.laxiflora,traditional Chinese herbal medicines,possesses strong antileukemic activity with low toxicity.In murine t(8;21)leukemia models,EriB remarkably prolong the survival time and decreased the xenograft tumor size by targeting AML1-ETO oncoprotein.In angiogenesis research by the highly vascularized chorioallantoic membrane(CAM)of the chicken embryo further confirm its antiangiogenic activity.Microarray offers a high efficient approach to study the gene expression profile treated by EriB,so as to provide systematic information about potential mechanisms of EriB curing AML.METHODS The t(8;21)AML cell line Kasumi-1 is most sensitive to EriB.Cells are treated with Eri-B(0.5μmol·L-1)and collected in 2,6,12 and 24h,respectively.Using human cDNA microarray,we investigate the changes of differential gene expression of Kasumi-1cells by time before and after Eri-B treatment.Meanwhile,the mRNA expression of TRAF2,DEDD2,BAG3,SAT1,IQGAP1,C-myc and GRB2 is detected by semi-quantitative RT-PCR and real-time quantitative PCR.RESULTS The genes regulated significantly are correlated with cell proliferation,apoptosis,cell circle,regulation of transcription,response to stimulies and metabolism.Regulation of TNFR mediated apoptotic signaling by EriB plays a key role to induce apoptosis and cell circle,involved NF-κB,Ras-MAPK,cAMP/PKA,PI3K/Akt,Cyt c/caspase and p53-Rb signal pathways.After detected by SqRT-PCR and real-time quantitative PCR,the mRNA expressions of BAG3,DEDD2,SAT1 and C-myc are significantly changed.CONCLUSION These findings describes the probable mechanisms involved and the value of EriB as a promising candidate targeting apoptosis cascade and cell circle in treatment of AML.
