＜正＞Real-time quantitative reverse transcription PCR （qRT-PCR） data needs to be normalized for its proper inter...
Ruibo Hu1, Chengming Fan1, Hongyu Li1, Qingzhu Zhang1, Chentao Lin1,2*, Yongfu Fu1* 1Institute of Crop Sciences, National Key Facility of Crop Gene Resource and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China 2Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA
＜正＞Photoperiodic control of flowering time is believed to affect latitudinal distribution of plants. The blue ...
Qingzhu Zhang1,2,*, Hongyu Li1,*, Rui Li1,*, Ruibo Hu1, Chengming Fan1, Fulu Chen1, Zonghua Wang2, Xu Liu1,Yongfu Fu1,2, and Chentao Lin1,c,2 1Institute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China
＜正＞The Arabidopsis genome encodes 29 AHL （AT-hook motif nuclear localized） proteins, but the function for most...
Chaowen Xiao1,3, Fulu Chen1,3, Xuhong Yu2,3, Chentao Lin2,*, Yong-Fu Fu1,* 1Institute of Crop Sciences, The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Chinese Academy of Agricultural Sciences, Nandajie12, Zhongguancun, Haidian District, Beijing 100081, P.R. China 2Department of Molecular, Cell & Developmental Biology, University of California, Los Angeles, CA90095
大豆(Glycine max L.)是典型的短日照植物,光周期反应对开花至关重要。GmLCL2可能是大豆生物钟中的生物振荡器关键基因。为进一步研究GmLCL2基因的功能及与其他生物钟基因的相互作用,本试验克隆了GmLCL2的特异片段,制备了多克隆抗体并对细菌及大豆不同组织进行了免疫印迹检测。试验结果表明,多克隆抗体效价为1∶2000,并且在大豆不同组织(上胚轴、下胚轴、根、茎尖生长点和叶片)中均检测到GmLCL2蛋白的特异表达。获得的GmLCL2多克隆抗体效价较好,特异性较强,为后续GmLCL2功能研究提供良好探针。
