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国家自然科学基金(30600669)

作品数:6 被引量:12H指数:2
相关作者:朱兰郎景和刘海元孙大为冷金花更多>>
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子宫内膜异位症患者血浆蛋白质指纹图谱诊断分类树模型的初步建立被引量:6
2009年
目的应用表面增强激光解吸电离飞行时间质谱(SELDI—TOF—MS)技术结合生物信息学方法筛选子宫内膜异位症(内异症)患者的血浆生物学标志物,并初步建立内异症患者血浆蛋白质指纹图谱诊断分类树模型。方法收集2007年1—10月北京协和医院妇产科收治的因内异症而行腹腔镜手术的内异症患者36例(内异症组)及因卵巢良性肿瘤和不孕行腹腔镜手术的非内异症患者35例(对照组)。采用SELDI—TOF—MS技术及其配套蛋白质芯片检测两组患者的血浆蛋白质指纹图谱,比较两组蛋白质峰的差异。采用分类与回归树(CART)软件建立内异症诊断分类树模型,并对该模型诊断内异症的敏感度和特异度进行验证。结果与对照组比较,内异症组患者血浆中有14个异常表达的蛋白质峰(P〈0.01)。采用相对分子质量分别为3956000、11710000和6986000的3个蛋自质峰构成的内异症诊断模型,其敏感度为92%,特异度为83%;验证后其敏感度为88%,特异度为80%。结论SELDI—TOF—MS技术对于筛选内异症的生物标志物提供了一条新的途径,其临床应用价值值得进一步研究。
刘海元郑艳华张建中冷金花孙大为刘珠凤朱兰郎景和
关键词:子宫内膜异位症血蛋白质类肽谱基质辅助激光解吸电离
Primary establishment of human uterine muscle proteomic profiling by two-dimensional electrophoresis and mass spectrometry
2008年
刘海元郎景和刘珠凤朱兰冷金花孙大为王晓荣
关键词:肌肉组织学蛋白组学双向电泳
Increased expression of stathmin in eutopic endometrium of patients with endometriosis被引量:2
2010年
Background Stathmin was identified as an endometriosis-related protein by comparative proteomics in our previous study.As a microtubule-destablizing factor, stathmin was shown to participate in the relay and integration of diverse intracellular signaling pathways involved in cell proliferation, differentiation, and many other cellular activities.To investigate whether stathmin is involved in the pathogenesis of endometriosis, we examined the expression of stathmin in eutopic endometrium of women with or without endometriosis.Methods Eutopic endometrium samples were collected from thirty-six patients who were diagnosed as endometriosis and the nineteen age-matched patients who were confirmed to be free of endometriosis surgically and histologically.The expression of stathmin mRNA was detected by real-time PCR, and its protein was detected by Western blotting and immunohistochemistry.Results Stathmin was overexpressed in eutopic endometrium of women with endometriosis detected by real-time PCR in mRNA levels and by Western blotting in protein levels, without significant difference between proliferative and 0secretory phase.Immunohistochemistry showed that stathmin protein was localized in both endometrial glandular and stromal cells throughout the menstrual cycle.Conclusions Stathmin is overexpressed in endometrium of patients with endometriosis and may play a role in the pathogenesis of endometriosis.
LI Chun-yanLIU Hai-yuanLANG Jing-heWANG Hong-qingFAN Xiu-ling
关键词:STATHMINENDOMETRIOSIS
Expression of Annexin-1 in patients with endometriosis被引量:2
2008年
Background Annexin-1 was identified as an endometriosis-related protein by comparative proteomics in previous study. As an endogeneous anti-inflammatory mediator, Annexin-1 has been shown to regulate the immune response, cell proliferation and apoptosis. To investigate whether Annexin-1 is involved in the pathogenesis of endometriosis, we examined the expression of Annexin-1 in eutopic endometrium of women with or without endometriosis, and detected its expression in peritoneal fluids of those with endometriosis. Methods Eutopic endometrium samples from twenty-five women with endometriosis and those from sixteen age-matched women without endometriosis were collected. Peritoneal fluids were obtained from ten patients with endometriosis. The expression of Annexin-1 protein in eutopic endometrium was detected by immunohistochemistry and Western blotting, and mRNA detected by real-time PCR. Annexin-1 protein in the peritoneal fluids was detected by Western blotting.Results Annexin-1 mRNA and protein were overexpressed in eutopic endometrium of endometriosis without significant differences between the proliferative and secretory phase. Immunohistochemistry showed that Annexin-1 protein was expressed mainly in endometrial glandular celts throughout the menstrual cycle. Annexin-1 protein was detected in the peritoneal fluids of all the ten patients with endometriosis. Conclusions Annexin-1 is overexpressed in eutopic endometrium and presents in the peritoneal fluids of patients with endometriosis, and may play a role in the pathogenesis of endometriosis.
LI Chun-yan LANG Jing-he LIU Hai-yuan ZHOU Hui-mei
关键词:ANNEXIN-1ENDOMETRIOSISENDOMETRIUM
人正常子宫内膜蛋白质表达图谱的初步建立
2008年
目的通过双相电泳方法建立正常子宫内膜组织的蛋白质表达图谱。方法收集正常卵泡期子宫内膜组织5份,采用双相电泳建立子宫内膜组织的蛋白质表达谱;利用凝胶染色技术和图像分析软件进行蛋白质点的识别和检测;随机选择5个蛋白质点,应用基质辅助激光解析离子化飞行时间质谱(MALDI-TOF-MS)和生物信息学方法进行蛋白质的鉴定。结果以固相pH梯度(IPG)胶条pH=4~9进行第一相等电聚焦,以12.5%SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)为第二相,成功建立了正常卵泡期子宫内膜组织蛋白质双相电泳图谱,平均含(523±41)个蛋白点,匹配率81.89%。通过MALDI—TOF-MS对5个蛋白质点进行了鉴定。结论成功建立了人正常子宫内膜蛋白质双相电泳图谱,并应用质谱技术进行了部分蛋白质点的鉴定,为进一步构建人子宫内膜蛋白质表达数据库提供了基础,并为研究子宫内膜生理功能和相关疾病提供了一种新的研究方法。
刘海元郎景和冷金花孙大为朱兰刘珠凤
关键词:子宫内膜双相电泳蛋白质组
子宫腺肌病的比较蛋白质组学研究被引量:2
2008年
目的建立子宫腺肌病病灶组织的蛋白质表达图谱,筛选并鉴定差异表达的蛋白质。方法收集2007年1月至10月北京协和医院妇产科收治的因子宫腺肌病行子宫全切除术患者的子宫肌层病灶组织(腺肌病组)及因宫颈上皮内瘤变和宫颈癌行子宫全切除术患者的正常子宫肌层组织(对照组)各5份,采用双向凝胶电泳技术分别建立两组的蛋白质表达图谱;利用图像分析软件进行比较分析,寻找差异表达的蛋白质点;应用基质辅助激光解吸电离飞行时间质谱和生物信息学方法鉴定差异表达的蛋白质,并进行功能分析。结果子宫腺肌病组织考马斯亮蓝染色图谱中平均含(512±36)个蛋白质点,以其中1块凝胶为参考胶进行匹配,不同凝胶之间蛋白质点的匹配率为83.7%;银染图谱中平均含(762±54)个蛋白质点,不同凝胶之间蛋白质点的匹配率为81.1%。与对照组比较,腺肌病组中有15个恒定差异表达的蛋白质点,其中10个被成功鉴定。这些蛋白质的功能涉及细胞骨架、氧化反应、凋亡和免疫反应等。结论子宫腺肌病组织在细胞骨架、氧化反应、凋亡、免疫反应等过程中存在异常,这些过程可能参与了子宫腺肌病的发病。
刘海元冷金花孙大为朱兰刘珠凤郎景和王晓荣武淑珍
关键词:子宫内膜异位症光谱法基质辅助激光解吸电离蛋白质组学
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