Nitric oxide was discovered in both the lab and the alga culture pond of Daya Bay (1―300 m3) before the growth of alga reached the maximum. The results included: (1) NO was detected before the growth of alga reached the maximum in the case of red tide alga and food alga, and the concentration of NO decreased rapidly after the growth maximum; (2) the curve between NO con-centration and time indicated that the concentration of NO in the daytime was more than that at night, and the maximal concentration of NO appeared in the midday (1―3 pm); (3) the growth of alga reached the maximum in the alga culture pond of Daya Bay in about 8―10 d, and NO was discovered in 5―7 d; (4) the measured NO concentration was 10-9 mol/L, 10-9―10-8 mol/L, and 10-8 mol/L for Haeterosigma akashiwo, mixed alga in Daya Bay and Chaetoceros Curvisetus individually; (5) the relation of illumination with NO production was discussed.
ZHANG Zhengbin XING Lei WU Zhenzhen LIU Chunying LIN Cai LIU Liansheng
Prorocentrum micans was cultivated in different media including f/2, f/4, f/8, f/20, f/25, f/50, f/100 and f/200. Results showed that media influence the growth of P.micans. The biomass of P.micans in rich nutrition medium was much higher than in poor nutrition medium. Nitric oxide can promote or inhibit the growth of P.micans in all media. Nitric oxide at the concentration of 1.4×10 -6 mol L -1 promoted the growth of P.micans significantly when added only once during the cultivation. When added twice a day, nitric oxide at the concentration of 1.4×10 -9 mol [KG-*4]L -[KG-*5]1 promoted the growth of P.micans significantly, while nitric oxide at the concentrations of 1.4×10 -5 mol L -1 and 1.4×10 -6 mol L -1 inhibited the growth. Therefore, nitric oxide, media and the ways to add nitric oxide influenced the growth of P.micans respectively.
Different from the solution/physical pump, biological pump and continental shelf pump of carbon cycle in oceans, a new pump named “surface microlayer (SML) pump” is developed based on data obtained from marine investigations and lab study. The SML pump has: (1) left-right dissymmetry of “pH-depth” curve; (2) the non-linearity of “concentration-depth” curve; and (3) difference of affect-ing confine of the SML pump. The issue of “source” or “sink” of atmospheric CO2 in the Yellow Sea and South China Sea is discussed.
ZHANG Zhengbin GONG Haidong LIU Liansheng ZHANG Chuang
