Anti-HER2 monoclonal antibody(Sc7301)-paclitaxel(TAX)immunoconjugate was prepared and its specific binding to tumor cells was investigated in this study.Sc7301 was conjugated to TAX by the active ester method and then the TAX-Sc7301 immunoconjugate was obtained.After purification and labeling by Cyano-fluorescein isothiocyanate(FITC),the specific binding of TAX-Sc7301 to HER2-positive tumor cells(SKOV3)and HER2-negative tumor cells(HepG2)was evaluated respectively.TAX-Sc7301(20 nmol/L)showed distinct specific binding to SKOV3 cells rather than HepG2 cells.And the uptake of the immunoconjugate by SKOV3 cells was increased with the TAX-Sc7301 concentration(3-48 nmol/L)and the incubation time(P<0.05).It was concluded that the TAX-Sc7301 immunoconjugate is potentially applicable as a targeted agent against HER2-positive tumor cells.
