Objective:To explore the effect of Yiqi Huoxue Decoction on the metabolomics of acute myocardial infarction rats.Method:Left anterior descending coronary artery ligation was used to establish an acute myocardial infarction animal model.The rats were randomly divided into model,perindopril,QishenYiqi Dropping Pill and Yiqi Huoxue Decoction groups and compared with a shamoperation group.Blood metabolite changes were detected by nuclear magnetic resonance metabolomics 7 and 28 days after operation.Metabolite differences between the administration and model groups were analyzed.P-values of the differences in metabolite levels between the drug and model groups were statistically analyzed.Results:Compared with the sham-operation group,there were 22 altered metabolites in the model group identified after 7 days and 15 identified after 28 days.Products of energy,carbohydrate,lipid and amino acid metabolism were the primary metabolites observed in the analyzed samples.Conclusion:After intervention with traditional Chinese medicine,there were clear changes in energy,carbohydrate,lipid and amino acid metabolism that restored the metabolic phenotype to the normal range,thus repairing the damage caused by myocardial infarction.
Lu ZhangChenglong ZhengMin JiangWenchen LiuHui WangZhiyong WangJiani WuShuwen Guo
OBJECTIVE: To investigate the effect of Yiqihuoxue prescription(YQHX) from Traditional Chinese Medicine(TCM) on myocardial glucose and lipid metabolism after myocardial infarction via the cross talk between the liver kinase B1(LKB1)-dependent Notch1 and adenosine 5'-monophosphate(AMP)-activated protein kinase(AMPK). YQHX was prepared with substances with properties that benefit, to activate blood circulation based on the TCM theory.METHODS: Animal models of myocardial infarction were established by ligating Sprague Dawley rats' left anterior descending coronary arteries. The animals were randomly divided into a myocardial infarction(MI) group, a YQHX group, a perindopril group, a γ-secretase inhibitor, Notch signal inhibitor(DAPT) group, a DAPT+YQHX group and a sham group. The related drugs were administered on the second day after operation, and changes in the relevant indexes were examined on weeks 1 and 4.Changes in cardiac structure and function were examined by echocardiography. The glucose and free fatty acids(FFA) were examined by ELISA. The expression of Notch, LKB1 and AMPK m RNA was examined by a real-time fluorescence quantitative method. The expression of glucose transporter 4(GLUT4), and the expression of total acetyl-Co A carboxylase(ACC) and its phosphorylation were examined by western blotting.RESULTS: Compared with the sham group, the expression of Notch, LKB1 and AMPK m RNA in the MI group was lower. Compared with the MI group, the expression of these m RNAs in the YQHX and perindopril groups was higher, and their expression in the DAPT group was lower. At all time points, the protein expression of GLUT4 and p ACC decreased in the MI group. On week 1, the expression of p ACC protein was higher. In the DAPT group, the expression of p ACC protein decreased. Compared with the YQHX group, the expression of p ACC protein in the DAPT + YQHX group was lower. On week 4,compared with the MI group, the expression of GLUT4 protein in the YQHX group and the perindo-pril group was higher. The expression of
