目的：利用Bioinformatics Analysis Tool for Molecular mech ANism of Traditional Chinese Medicine（BATMAN-TCM）在线分析工具预测玄胡索散治疗骨关节炎（osteoarthritis,OA）作用并进行初步试验验证。方法：在数据库中检索玄胡索散中单味药化学成分信息,利用BATMAN-TCM预测其潜在作用靶点、通路与疾病,构建化学成分-靶点-疾病网络,预测其治疗OA的作用。采用前交叉韧带横断联合半月板切除术造OA大鼠模型,经玄胡索散治疗后观察其对OA大鼠膝关节组织病理学与总胶原的影响,初步验证预测结果。结果：经BATMANTCM预测发现玄胡索散能作用于多条与OA相关通路,并具有治疗多种骨相关疾病的功效,动物试验表明玄胡索散能明显抑制OA大鼠膝关节软骨损伤（P〈0.05）与胶原降解（P〈0.05）。结论：BATMAN-TCM预测结果较为可靠,玄胡索散具有治疗OA的潜在作用。
The present study aimed to establish a pharmacodynamic method using the py Solo software to explore the influence of freeze-dried powders of Shuangxia Decoction(SXD) on the sleep of normal Drosophila melanogaster and the Drosophila melanogaster whose sleep was divested by light. The dose-effect and the time-effect relationships of SXD on sleep were examined. The effect-onset concentration of SXD was 0.25%, the plateau appeared at the concentration of 2.5% and the total sleep time showed a downtrend when the concentration was greater than 2.5%. The sleep time was the longest on the fourth day after SXD was given. The fruit fly sleep deprivation model was repeated by light stimulation at night. The middle dosage group(2.5%) had the best insomnia-curing effect. In conclusion, using the py Solo software, an approach for the pharmacodynamics study was established with Drosophila melanogaster as a model organism to determine the insomnia-curing effects of the traditional Chinese medicine(TCM). Our results demonstrated the reliability of this method. The freeze-dried powders of SXD could effectively improve the sleep quality of Drosophila melanogaster.
